诱变活性作为河流水污染指示物的有效性。
Validity of mutagenic activity as an indicator of river water pollution.
机构信息
Fukuoka Institute of Health and Environmental Sciences, 39 Mukaizano, 818-0135, Dazaifu, Fukuoka, Japan.
出版信息
Environ Health Prev Med. 2003 Sep;8(4):133-8. doi: 10.1007/BF02897917.
OBJECTIVES
To investigate if mutagenicity could be expressed by known water pollution indicators, we determined the mutagenic activity of blue rayon extracts from sampled river water with the Ames test utilizing new strains of bacteria, and compared the results with those of known indicators of water pollution.
METHODS
Water samples were collected by the blue rayon adsorption method at sixteen sites in six rivers in the North Kyushu district. The Assay of mutagenicity was carried out using the Ames test. The test strains wereSalmonella typhimurium TA100, YG1024, YG1041 and YG1042. B(a)P, Trp-P-1 and Trp-P-2 were quantified by HPLC. Determinations of SS, BOD, COD, T-N, T-P, DOC, and A(260)/DOC were performed.
RESULTS
The extracts from five sampling sites showed higher mutagenicity toward strain YG1024 with or without S9mix, and the extracts from two of these five sites showed higher mutagenicity toward strain YG1041 with and without S9mix. However, the water pollution indicators did not show specific trends that were consistent with the mutagenic activity.
CONCLUSIONS
Since the mutagenic activity of river water could not be predicted using known water pollution indicators, we recommend that biological examinations such as mutagenicity tests be added to the indicators that are currently in use.
目的
为了研究是否可以通过已知的水污染指标来表达致突变性，我们利用新的细菌菌株，利用 Ames 试验来测定从采样河流水中提取的蓝色人造丝的致突变活性，并将结果与已知的水污染指标进行比较。
方法
通过蓝色人造丝吸附法在北九州地区的六条河流的十六个地点采集水样。采用 Ames 试验进行致突变性测定。试验菌株为鼠伤寒沙门氏菌 TA100、YG1024、YG1041 和 YG1042。通过 HPLC 定量测定 B(a)P、Trp-P-1 和 Trp-P-2。测定 SS、BOD、COD、T-N、T-P、DOC 和 A(260)/DOC。
结果
五个采样点的提取物对 YG1024 菌株具有更高的致突变性，无论是否添加 S9mix，而这五个点中的两个提取物对 YG1041 菌株具有更高的致突变性，无论是否添加 S9mix。然而，水污染指标并没有表现出与致突变活性一致的特定趋势。
结论
由于不能使用已知的水污染指标来预测河水的致突变活性，因此我们建议在当前使用的指标中增加如致突变性测试等生物检测。
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