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表皮葡萄球菌的一种炎症性多肽复合物：分离与特性鉴定

An inflammatory polypeptide complex from Staphylococcus epidermidis: isolation and characterization.

作者信息

Mehlin C, Headley C M, Klebanoff S J

机构信息

Department of Pathobiology, University of Washington, Seattle, Washington 98195, USA.

出版信息

J Exp Med. 1999 Mar 15;189(6):907-18. doi: 10.1084/jem.189.6.907.

DOI:10.1084/jem.189.6.907
PMID:10075974
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2193041/
Abstract

Staphylococcus epidermidis releases factors that activate the HIV-1 long terminal repeat, induce cytokine release, and activate nuclear factor B in cells of macrophage lineage. The active material had a mass of 34,500 daltons, was inactivated by proteases and partitioned into the phenol layer on hot aqueous phenol extraction, and thus was termed phenol-soluble modulin (PSM). High performance liquid chromatography (HPLC) of crude PSM yielded two peaks of activity designated PSM peak 1 and peak 2. MALDI-TOF (matrix-assisted laser desorption ionization-time of flight) mass spectroscopy indicated the presence of two components in peak 1, which were designated PSM and PSM. Peak 2 contained a single component, designated PSM. Separation of PSM and PSM in peak 1 could be achieved by a second HPLC procedure. The structure of each component was determined by amino acid sequence analysis and identification and sequencing of their genes. PSM, PSM, and PSM were 22-, 44-, and 25-amino acid, respectively, strongly hydrophobic polypeptides. PSM was identified as Staphylococcus epidermidis delta toxin, whereas PSM and PSM exhibited more distant homology to previously described staphylococcal toxins. They appeared to exist as a complex or aggregate with activity greater than the component parts. The properties of the S. epidermidis PSMs suggest that they may contribute to the systemic manifestations of Gram-positive sepsis.

摘要

表皮葡萄球菌释放的因子可激活HIV-1长末端重复序列、诱导细胞因子释放并激活巨噬细胞系细胞中的核因子κB。活性物质的分子量为34,500道尔顿，可被蛋白酶灭活，在热酚水提取时分配到酚层中，因此被称为酚溶性调节素（PSM）。粗制PSM的高效液相色谱（HPLC）产生了两个活性峰，分别命名为PSM峰1和峰2。基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱表明峰1中存在两种成分，分别命名为PSMα和PSMβ。峰2包含单一成分，命名为PSMγ。通过第二次HPLC程序可实现峰1中PSMα和PSMβ的分离。通过氨基酸序列分析以及它们基因的鉴定和测序确定了每种成分的结构。PSMα、PSMβ和PSMγ分别为22、44和25个氨基酸的强疏水性多肽。PSMα被鉴定为表皮葡萄球菌δ毒素，而PSMβ和PSMγ与先前描述的葡萄球菌毒素表现出更远的同源性。它们似乎以复合物或聚集体的形式存在，其活性大于各组成部分。表皮葡萄球菌PSM的特性表明它们可能导致革兰氏阳性败血症的全身表现。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6830/2193041/8dde12aa1e59/JEM981305.f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6830/2193041/f0afc0786dec/JEM981305.f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6830/2193041/e82b44873047/JEM981305.f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6830/2193041/9e843247ffac/JEM981305.f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6830/2193041/02f940e8f4ec/JEM981305.f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6830/2193041/d522df802e32/JEM981305.f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6830/2193041/347b7dac4c87/JEM981305.f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6830/2193041/e05d38742b01/JEM981305.f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6830/2193041/2729dffcb73c/JEM981305.f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6830/2193041/382ad935f671/JEM981305.f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6830/2193041/8dde12aa1e59/JEM981305.f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6830/2193041/f0afc0786dec/JEM981305.f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6830/2193041/e82b44873047/JEM981305.f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6830/2193041/9e843247ffac/JEM981305.f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6830/2193041/02f940e8f4ec/JEM981305.f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6830/2193041/d522df802e32/JEM981305.f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6830/2193041/347b7dac4c87/JEM981305.f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6830/2193041/e05d38742b01/JEM981305.f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6830/2193041/2729dffcb73c/JEM981305.f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6830/2193041/382ad935f671/JEM981305.f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6830/2193041/8dde12aa1e59/JEM981305.f10.jpg

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