Endo S, Inada K, Nakae H, Arakawa N, Takakuwa T, Yamada Y, Shimamura T, Suzuki T, Taniguchi S, Yoshida M
Critical Care and Emergency Center, Iwate Medical University, Morioka, Japan.
Res Commun Mol Pathol Pharmacol. 1996 Mar;91(3):347-56.
To investigate the involvement of nitrite/nitrate oxide (NOx) in septic shock, and to evaluate the relationships between NOx and cytokines in patients with this disorder, we evaluated 11 patients with septic shock and 12 patients with sepsis unassociated with shock. NOx were measured with an automated system based on the Griess reaction. The plasma concentrations of various cytokines were determined by enzyme-linked immunosorbent assay. Endotoxin was determined by a specific assay after the plasma samples were processed by a perchloric acid method. The mean plasma levels of NOx in the group with shock significantly exceeded those in the group without shock. Significant correlations were observed between the plasma levels of NOx and those of endotoxin, tumor necrosis factor-alpha, and interleukin 8 in both groups. NOx appeared to be involved in the development of septic shock in humans. Endotoxin and cytokines appeared to be involved in the production of NOx.
为了研究亚硝酸盐/一氧化氮（NOx）在感染性休克中的作用，并评估NOx与该疾病患者细胞因子之间的关系，我们对11例感染性休克患者和12例无休克的脓毒症患者进行了评估。采用基于格里斯反应的自动化系统测定NOx。通过酶联免疫吸附测定法测定各种细胞因子的血浆浓度。血浆样本经高氯酸法处理后，通过特异性测定法测定内毒素。休克组的平均血浆NOx水平显著高于无休克组。两组中NOx的血浆水平与内毒素、肿瘤坏死因子-α和白细胞介素8的血浆水平之间均存在显著相关性。NOx似乎参与了人类感染性休克的发生发展。内毒素和细胞因子似乎参与了NOx的产生。
