Bakalova R, Goudev A, Zhelev Z, Nachev C
Department of Medical Physics and Biophysics, High University School of Medicine, Sofia, Bulgaria.
Acta Physiol Pharmacol Bulg. 1995;21(4):81-5.
Oxidative modifications of blood serum in humans with and without coronary artery disease were investigated. Four parameters were analyzed: the intensity of serum fluorescence, which is indicative of the content of lipofuscine-like lipid peroxidation products; the content of thiobarbituric acid-reactive substances; the lag-phase of serum oxidation by azo-compounds; and the content of lipophilic natural antioxidants--alpha-tocopherol, beta-carotene and ubiquinol-9(10). It was found that coronary artery disease resulted in a significant increase of serum fluorescence and the content of TBARS. The atherogenic disorders in humans with coronary artery disease drastically decreased the lag-phase of serum oxidation in the presence of 2,2'-azo-bis-(2-amidinopropane) dihydrochloride. The oxidative modifications of serum were in close correlation with the balance of natural lipophilic antioxidants in blood serum, i.e. alpha-tocopherol, ubiquinols and beta-carotene. The contents of all antioxidants tested in serum were significantly decreased in patients with coronary artery disease.
对患有和未患有冠状动脉疾病的人的血清氧化修饰进行了研究。分析了四个参数：血清荧光强度，其指示类脂褐素样脂质过氧化产物的含量；硫代巴比妥酸反应性物质的含量；血清被偶氮化合物氧化的滞后期；以及亲脂性天然抗氧化剂——α-生育酚、β-胡萝卜素和泛醇-9(10)的含量。结果发现，冠状动脉疾病导致血清荧光和TBARS含量显著增加。患有冠状动脉疾病的人的动脉粥样硬化紊乱在存在2,2'-偶氮双-(2-脒基丙烷)二盐酸盐的情况下显著缩短了血清氧化的滞后期。血清的氧化修饰与血清中天然亲脂性抗氧化剂即α-生育酚、泛醇和β-胡萝卜素的平衡密切相关。在患有冠状动脉疾病的患者中，血清中所有测试抗氧化剂的含量均显著降低。
