Oteiza P I, Uchitel O D, Carrasquedo F, Dubrovski A L, Roma J C, Fraga C G
Departmento de Química Biológica (IQUIFIB-CONICET), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Neurochem Res. 1997 Apr;22(4):535-9. doi: 10.1023/a:1027384432715.
Several parameters indicators of oxidative stress were evaluated in blood from individuals with the sporadic form of amyotrophic lateral sclerosis (SALS) and compared to healthy controls. Plasma levels of 2-thiobarbituric-reactive substances (TBARS), products of lipid peroxidation, were significantly higher (p < 0.03) in the SALS patients compared to controls. The concentration of plasma antioxidants (alpha-tocopherol, beta-carotene, ubiquinol-10 and glutathione) and the activity of red blood cell CuZn superoxide dismutase were not significantly different between the groups. The ratio TBARS/alpha-tocopherol was 47% higher in the SALS individuals than in controls. Protein thiols and protein-associated carbonyls in red blood cell membranes and supernates were similar for both groups. A positive correlation (r2 = 0.91) was found between the concentration of protein-associated carbonyls in red blood cells and the onset of clinical symptoms. These findings are in agreement with several reports showing higher levels of oxidative damage to cell components in ALS.
对散发性肌萎缩侧索硬化症（SALS）患者血液中的氧化应激相关参数指标进行了评估，并与健康对照组进行了比较。脂质过氧化产物2-硫代巴比妥酸反应性物质（TBARS）的血浆水平在SALS患者中显著高于对照组（p < 0.03）。两组之间血浆抗氧化剂（α-生育酚、β-胡萝卜素、泛醇-10和谷胱甘肽）的浓度以及红细胞铜锌超氧化物歧化酶的活性无显著差异。SALS患者的TBARS/α-生育酚比值比对照组高47%。两组红细胞膜和上清液中的蛋白质巯基和蛋白质相关羰基相似。红细胞中蛋白质相关羰基的浓度与临床症状的发作之间存在正相关（r2 = 0.91）。这些发现与多项报告一致，这些报告显示ALS患者细胞成分的氧化损伤水平较高。
