Gruys E, Castaño M
Vet Pathol. 1977 Jul;14(4):407-19. doi: 10.1177/030098587701400413.
Vacuoles of mesenchymal cells in the papillae of bovine kidneys with amyloidosis were studied by histochemical electron microscopy for acid phosphatase as a marker for lysosomes. The vacuoles contained parallel amyloid fibrils. The vacuoles of reticular interstitial cells were found to be lysosomes. Vacuoles of macrophage-like cells of the same papillae were positive, partially positive, or negative for the enzyme activity. A suspension of papillary material was injected subcutaneously in rats in a 21-day light and electron microscopy and enzyme histochemistry study. Amyloid was demonstrated in vacuoles of macrophages throughout this period and initially also in neutrophils. In most vacuoles amyloid fibrils were randomly arranged but in others parallel arrangement was demonstrated. Amyloid was only at the inoculation sites. Intralysosomal bovine amyloid may occur in parallel fibrillar arrangement without a definite indication for amyloid production.
通过组织化学电子显微镜，以酸性磷酸酶作为溶酶体的标志物，对患淀粉样变性的牛肾乳头间充质细胞的液泡进行了研究。这些液泡含有平行的淀粉样纤维。发现网状间质细胞的液泡是溶酶体。同一乳头的巨噬细胞样细胞的液泡对酶活性呈阳性、部分阳性或阴性。在一项为期21天的光镜、电镜和酶组织化学研究中，将乳头物质的悬浮液皮下注射到大鼠体内。在此期间，巨噬细胞的液泡中均显示有淀粉样物质，最初在中性粒细胞中也有。在大多数液泡中，淀粉样纤维呈随机排列，但在其他液泡中则显示为平行排列。淀粉样物质仅出现在接种部位。溶酶体内的牛淀粉样物质可能以平行纤维状排列出现，而没有明确的淀粉样物质产生迹象。
