Petri G, Krawczyk U, Kéry A
Semmelweis Orvostudományi Egyetem Gyógynövény- és Drogismereti Intézet, Budapest.
Acta Pharm Hung. 1994 Jul;64(4):117-22.
Analysis of the bilberry anthocyanin extracts of different origin (French, Polish, Roumanian and Italian) was performed for qualification and identification. Direct spectrophotometry was considered as simple, informative method for controlling the purity of the extracts and proving the presence of polyphenols with the estimation of the ratio A1 cm1% 535/A1 cm1% 280. Thin-layer chromatography is an appropriate technique for the separation of 5 to 6 constituents in the extracts for qualification and for the evaluation of compound-ratios by TLC-densitometry. The new RP-HPLC gradient procedure seemed to be the best method for the standardization of bilberry extracts by the separation and quantitative estimation of 12 anthocyanins. Therefore it is of great value in the standardization of anthocyanin extracts and crude drugs containing them.
对不同产地（法国、波兰、罗马尼亚和意大利）的越橘花青素提取物进行了定性和鉴定分析。直接分光光度法被认为是一种简单、有效的方法，可用于控制提取物的纯度，并通过估算A1 cm1% 535/A1 cm1% 280的比值来证明多酚的存在。薄层色谱法是一种合适的技术，可用于分离提取物中的5至6种成分以进行定性，并通过薄层色谱密度法评估化合物比例。新的反相高效液相色谱梯度程序似乎是通过分离和定量测定12种花青素对越橘提取物进行标准化的最佳方法。因此，它在花青素提取物和含有它们的粗药物的标准化方面具有重要价值。
