Weber L, Meigel W N, Spier W
Arch Dermatol Res. 1978 Feb 15;261(1):63-71. doi: 10.1007/BF00455376.
The collagen type composition of normal and pathologic scars was examined in comparison with normal skin from the same individual. Particular care was taken to separate scar tissue from adjacent normal dermis. After urea extraction, the tissue specimens were cleaved with cyanogen bromide. The presence of the dermal collagen types I and III was deduced from the electrophoretic distribution patterns of the CNBr peptides in 12% SDS-polyacrylamide gels. The intensity of the type III specific peptide bands correlates with the type III content of the samples. Using this method, the presence of both type I and III collagen can be proved in normal as well as pathologic scars. The type III content in older normal scars is slightly increased, whereas the type III content of pathologic scars is significantly increased in comparison with the type III content of normal skin. The electrophoretic CNBr peptide distribution pattern of pathologic scar tissue is almost the same as that of fetal skin. Both are clearly different from the peptide pattern of normal adult skin.
对正常瘢痕和病理性瘢痕的胶原类型组成进行了检测，并与同一个体的正常皮肤作比较。特别注意将瘢痕组织与相邻的正常真皮分离。尿素提取后，用溴化氰裂解组织标本。通过12%十二烷基硫酸钠-聚丙烯酰胺凝胶中溴化氰肽的电泳分布模式推断真皮I型和III型胶原的存在。III型特异性肽带的强度与样品中III型胶原的含量相关。使用该方法，可以证明正常瘢痕和病理性瘢痕中均存在I型和III型胶原。较陈旧的正常瘢痕中III型胶原含量略有增加，而病理性瘢痕中III型胶原含量与正常皮肤相比显著增加。病理性瘢痕组织的电泳溴化氰肽分布模式与胎儿皮肤几乎相同。两者均明显不同于正常成人皮肤的肽模式。
