Hubálek Z, Anderson J F, Halouzka J, Hájek V
Institute of Landscape Ecology, Academy of Sciences, Brno, Czech Republic.
J Med Entomol. 1996 Sep;33(5):766-71. doi: 10.1093/jmedent/33.5.766.
Of 411 forest birds of 33 species examined near Valtice, Czech Republic, 29% were infested with Ixodes ricinus (L.); 2.2% were parasitized by Haemaphysalis concinna Koch. Borreliae were detected in 5.1 and 11.7% of larval and nymphal I. ricinus, respectively. None of the 13 H. concinna tested was infected. In total, 3.2% of the birds examined were parasitized by I. ricinus immatures infected by borreliae. Borreliae-containing ticks parasitized European robin, Erithacus rubecula (L.); Eurasian blackbird, Turdus merula L.; Blackcap, Sylvia atricapilla (L.); Eurasian chiffchaff, Phylloscopus collybita (Vieillot); Great tit, Parus major L.; and Eurasian jay, Garrulus glandarius (L.). The isolate BR-34 from a nymphal I. ricinus off a Eurasian blackbird had a sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein pattern, reactions to monoclonal antibodies, and fragments of HindIII digested DNA probed with fla and ospA genes that suggested to us that it belongs to the genospecies Borrelia garinii. Free-living birds may be involved in the circulation of B. burgdorferi sensu lato principally as disseminators of infected ixodid ticks to new area.
在捷克共和国瓦尔蒂采附近检查的33种411只森林鸟类中，29%感染了蓖麻硬蜱（Ixodes ricinus (L.)）；2.2%被康氏血蜱（Haemaphysalis concinna Koch）寄生。在蓖麻硬蜱的幼虫和若虫中，分别有5.1%和11.7%检测到疏螺旋体。所检测的13只康氏血蜱均未感染。总体而言，所检查的鸟类中有3.2%被感染疏螺旋体的蓖麻硬蜱未成熟体寄生。携带疏螺旋体的蜱寄生在欧洲知更鸟（Erithacus rubecula (L.)）、欧亚黑鸫（Turdus merula L.）、黑头莺（Sylvia atricapilla (L.)）、欧亚 chiffchaff（Phylloscopus collybita (Vieillot)）、大山雀（Parus major L.）和欧亚松鸦（Garrulus glandarius (L.)）身上。从一只欧亚黑鸫身上采集的蓖麻硬蜱若虫分离出的BR - 34菌株，其十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳蛋白质图谱、对单克隆抗体的反应以及用fla和ospA基因探测的HindIII消化DNA片段表明，它属于基因种伽氏疏螺旋体（Borrelia garinii）。自由生活的鸟类可能主要作为感染的硬蜱向新区域的传播者参与广义伯氏疏螺旋体（Borrelia burgdorferi sensu lato）的传播。
