Ando K, Urano M, Nesumi N, Koike S
Cancer Res. 1977 Sep;37(9):3115-9.
The antitumor effect of anaerobic Corynebacterium liquefaciens was compared with that of specific immunization. Experimental tumors were fourth or fifth generation isotransplants of a NR-Sl squamous cell carcinoma that arose spontaneously in a C3Hf/He female mouse. Specific immunization failed to exhibit an antitumor effect, whereas a single administration of the bacterium markedly inhibited the growth of the tumor. This growth inhibition was most effective when C. liquefaciens was administered 2 to 4 days before transplantation of tumor cells, but marked inhibition was also observed when this agent was administered after transplantation. The inhibitory effect was independent of dose within a range of 0.1 to 2.0 mg/mouse; a single dose of less than 0.05 mg/mouse did not exhibit antitumor effect. Multiple administrations of large doses, if given with short treatment intervals, were no more effective than one small dose. Multiple doses given at 14-day intervals resulted in marked growth retardation. The dose of cells that produced 50% tumor takes in C. liquefaciens-treated animals was not significantly different from that in nontreated animals, indicating that this bacterium exhibited no lethal effect on the tumor cells studied.
将液化厌氧棒状杆菌的抗肿瘤作用与特异性免疫的抗肿瘤作用进行了比较。实验肿瘤是一只C3Hf/He雌性小鼠自发产生的NR-Sl鳞状细胞癌的第四代或第五代同基因移植瘤。特异性免疫未能表现出抗肿瘤作用，而单次给予该细菌则显著抑制了肿瘤的生长。当在肿瘤细胞移植前2至4天给予液化棒状杆菌时，这种生长抑制最为有效，但在移植后给予该制剂时也观察到了明显的抑制作用。在0.1至2.0mg/小鼠的范围内，抑制作用与剂量无关；单次剂量小于0.05mg/小鼠未表现出抗肿瘤作用。如果给药间隔短，多次给予大剂量并不比单次小剂量更有效。每隔14天给予多次剂量导致明显的生长迟缓。在接受液化棒状杆菌治疗的动物中产生50%肿瘤接种率的细胞剂量与未治疗动物中的剂量没有显著差异，表明该细菌对所研究的肿瘤细胞没有致死作用。
