Miyawaki H, Fujita J, Takigawa K, Negayama K, Yamagishi Y, Yamaji Y, Ouchi K, Nakazawa T, Kawanishi K, Takahara J
First Department of Internal Medicine, Kagawa Medical School, Yamaguchi, Japan.
Diagn Microbiol Infect Dis. 1995 Nov;23(3):77-83. doi: 10.1016/0732-8893(95)00176-x.
We used DNA fingerprinting by the arbitrarily primed polymerase chain reaction (AP-PCR) technique for an epidemiologic investigation of Pseudomonas cepacia nosocomial isolates obtained from patients attending our hospital. This approach was compared with conventional phenotypic typing and pulsed-field gel electrophoresis (PFGE). The patterns of gel electrophoresis of the products of AP-PCR differed significantly according to differences in the concentration of Mg2+ and in pH. AP-PCR and PFGE was identical in their resolving power, as the two methods generated four different profiles and identified the same group of strains. The AP-PCR method constitutes an easy alternative to the well-established PFGE method.
我们采用任意引物聚合酶链反应（AP-PCR）技术进行DNA指纹分析，以对我院患者分离出的洋葱伯克霍尔德菌医院感染菌株进行流行病学调查。该方法与传统的表型分型和脉冲场凝胶电泳（PFGE）进行了比较。AP-PCR产物的凝胶电泳图谱因Mg2+浓度和pH值的差异而有显著不同。AP-PCR和PFGE的分辨能力相同，因为这两种方法都产生了四种不同的图谱并鉴定出同一组菌株。AP-PCR方法是成熟的PFGE方法的一种简便替代方法。
