Liu P Y, Shi Z Y, Lau Y J, Hu B S, Shyr J M, Tsai W S, Lin Y H, Tseng C Y
Section of Infectious Diseases, Taichung Veterans General Hospital, Taiwan, Republic of China.
J Clin Microbiol. 1995 Dec;33(12):3304-7. doi: 10.1128/jcm.33.12.3304-3307.1995.
In this study, we evaluated three PCR methods for epidemiological typing of Burkholderia (Pseudomonas) cepacia--PCR-ribotyping, arbitrarily primed PCR (AP-PCR) and enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR)--and compared them with pulsed-field gel electrophoresis. The analysis was performed with 31 isolates of B. cepacia, comprising 23 epidemiologically unrelated isolates and 8 isolates collected from the same patient during two episodes of bacteremia. Pulsed-field gel electrophoresis, ERIC-PCR, and AP-PCR identified 23 distinct types among the 23 unrelated isolates, while PCR-ribotyping only identified 12 strain types, even after AluI digestion of the amplification products. Among the eight isolates collected from the same patient, all typing techniques revealed two clones of strains. The day-to-day reproducibilities of PCR-ribotyping and ERIC-PCR were good, while greater day-to-day variations were noted in the fingerprints obtained by AP-PCR. We conclude that all three PCR techniques are useful for rapid epidemiological typing of B. cepacia, but ERIC-PCR seems to be more reproducible and discriminative.
在本研究中，我们评估了三种用于洋葱伯克霍尔德菌（假单胞菌属）流行病学分型的聚合酶链反应（PCR）方法——PCR核糖体分型、任意引物PCR（AP-PCR）和肠杆菌重复基因间共有序列PCR（ERIC-PCR），并将它们与脉冲场凝胶电泳进行比较。分析使用了31株洋葱伯克霍尔德菌分离株，其中包括23株流行病学上不相关的分离株以及在两次菌血症发作期间从同一患者身上采集的8株分离株。脉冲场凝胶电泳、ERIC-PCR和AP-PCR在23株不相关分离株中鉴定出23种不同类型，而PCR核糖体分型即使在对扩增产物进行AluI酶切后也仅鉴定出12种菌株类型。在从同一患者采集的8株分离株中，所有分型技术均显示出两个菌株克隆。PCR核糖体分型和ERIC-PCR的日常重复性良好，而AP-PCR获得的指纹图谱显示出更大的日常变化。我们得出结论，所有三种PCR技术都可用于洋葱伯克霍尔德菌的快速流行病学分型，但ERIC-PCR似乎更具可重复性和鉴别力。
