van Houten J, Chang S Y, Kung C
Genetics. 1977 May;86(1):113-20. doi: 10.1093/genetics/86.1.113.
Six mutants of Paramecium tetraurelia with curious "Paranoiac" phenotypes have been isolated and examined. Instead of the normal transient avoiding reactions in Na+ solution, these mutants show "violent avoidances"--backing continuously for 10 to over 60 sec. This behavior corresponds to prolonged membrane excitation excitation.--Genetic analyses establish five genic loci at which mutations give the "Paranoiac" phenotype. Close linkage between two of these genes occurs. Allelic variants are found for two of the genes. In one case, the two alleles determine very different behavioral phenotypes ("Paranoiac" and "fast-2"). These results show that the mechanism(s) which shuts off excitation in the wild-type membrane is (are) complex, but in the future may be fruitfully pursued in mutants which are defective.
已分离并研究了六株具有奇特“偏执狂”表型的四膜虫突变体。这些突变体在Na+溶液中不会出现正常的短暂回避反应，而是表现出“剧烈回避”——持续后退10至60多秒。这种行为对应于膜兴奋的延长。——遗传分析确定了五个基因位点，在这些位点发生的突变会产生“偏执狂”表型。其中两个基因之间存在紧密连锁。发现了两个基因的等位变体。在一个案例中，这两个等位基因决定了非常不同的行为表型（“偏执狂”和“快速-2”）。这些结果表明，野生型膜中关闭兴奋的机制是复杂的，但未来在有缺陷的突变体中可能会有丰硕的研究成果。
