Ludwig M, Köchel H G, Fischer C, Ringert R H, Weidner W
Department of Urology, Justus-Liebig-Universität, Giessen, Germany.
Eur Urol. 1996;30(1):96-102. doi: 10.1159/000474152.
To evaluate the significance of HPV type 6b, 11, 16 and 18 together with type-specific antibodies in the serum of bladder carcinoma.
The prevalence of HPV type 6b, 11, 16 and 18 in bladder tumor, normal bladder and urethra together with type-specific antibodies in serum was investigated in 23 patients with bladder cancer and 9 patients with chronic cystitis. HPV DNA analysis was done by polymerase chain reaction (PCR). Open reading frames of HPV were expressed in Escherichia coli as beta-galactosidase fusion proteins.
HPV 6b was demonstrated in the tumor tissue of 6 patients (19%), and in the nonmalignant specimens of 6 further patients (19%). HPV 16/18 was only found in the urethral swabs of 2 patients (6%). Anti-HPV antibodies were positive in 7 patients (22%). There was no association between the demonstration of HPV 6b and the occurrence of bladder tumor in this study.
Though, in this study, HPV was not associated with bladder cancer, further investigation is necessary to elucidate the role of HPV 6b in bladder tissue possibly by a semiquantitative PCR in tissue samples and of anti-HPV antibodies in serum.
评估人乳头瘤病毒6b型、11型、16型和18型以及膀胱癌患者血清中各型特异性抗体的意义。
对23例膀胱癌患者和9例慢性膀胱炎患者的膀胱肿瘤、正常膀胱和尿道中的人乳头瘤病毒6b型、11型、16型和18型以及血清中的各型特异性抗体进行了研究。通过聚合酶链反应（PCR）进行人乳头瘤病毒DNA分析。人乳头瘤病毒的开放阅读框在大肠杆菌中表达为β-半乳糖苷酶融合蛋白。
6例患者（19%）的肿瘤组织中检测到了人乳头瘤病毒6b型，另有6例患者（19%）的非恶性标本中也检测到了该病毒。人乳头瘤病毒16/18型仅在2例患者（6%）的尿道拭子中发现。7例患者（22%）的抗人乳头瘤病毒抗体呈阳性。在本研究中，人乳头瘤病毒6b型的检测与膀胱肿瘤的发生之间没有关联。
虽然在本研究中，人乳头瘤病毒与膀胱癌无关，但仍有必要进一步研究，可能通过对组织样本进行半定量PCR以及检测血清中的抗人乳头瘤病毒抗体，来阐明人乳头瘤病毒6b型在膀胱组织中的作用。
