Clarke R M
J Anat. 1977 Jul;123(Pt 3):805-11.
Six groups of male Wistar rats were used, with mean weights of 29, 63, 97, 161, 249 and 399 g. Pieces of small intestine from three sites were examined after staining in bulk with the Feulgen reaction. Crypt/villus ratio (the number of crypts per villus) rose with age at all three sites, Villus height and crypt depth were measured on microdissected specimens. Villi in the proximal intestine were always taller than those distally. Proximal villi increased in height in successively older rats, except in the oldest group. Villi at the two distal sites tended to be tall in the youngest group of rats, but suffered a temporary reduction in height in the next two age groups. Crypt depth increased markedly within the first three age groups, and more slowly thereafter. Colchicine-metaphase accumulation rate was estimated from counts on microdissected intact crypts. The rate was low in the youngest group (8 cells/crypt/hour) but about 30 cells/crypt/hour in all other groups. After the changes during the early phase of rapid growth, no marked changes were seen during later life. The significance of these findings is discussed.
使用了六组雄性Wistar大鼠，平均体重分别为29、63、97、161、249和399克。用福尔根反应对来自三个部位的小肠组织进行整体染色后进行检查。隐窝/绒毛比（每个绒毛的隐窝数量）在所有三个部位均随年龄增长而升高，在显微解剖的标本上测量绒毛高度和隐窝深度。近端小肠的绒毛总是比远端的高。除最老的一组外，近端绒毛在年龄依次增大的大鼠中高度增加。在最年轻的大鼠组中，两个远端部位的绒毛往往较高，但在接下来的两个年龄组中高度暂时降低。隐窝深度在前三个年龄组中显著增加，此后增加较慢。秋水仙碱中期积累率通过对显微解剖的完整隐窝计数来估计。最年轻的组中该率较低（8个细胞/隐窝/小时），但在所有其他组中约为30个细胞/隐窝/小时。在快速生长的早期阶段发生变化后，在后期生活中未观察到明显变化。讨论了这些发现的意义。
