Hand A R, Oliver C
Histochem J. 1977 Jul;9(4):375-92. doi: 10.1007/BF01002972.
The structure and cytochemistry of GERL was studied in several different exocrine secretory cells, including the exorbital lacrimal gland, parotid, lingual serous (von Ebner's), submandibular, and sublingual salivary glands, and exocrine pancreas of the rat; the lacrimal, parotid and pancreas of the guinea-pig; and the lacrimal gland of the monkey. GERL was morphologically and cytochemically similar in all cell types studied. It was located in the inner Golgi region and consisted of cisternal and tubular portions. Immature secretory granules were in continuity with GERL through multiple tubular connections. Modified cisternae of endoplasmic reticulum, with ribosomes only on one surface, closely paralleled parts of GERL. GERL and immature granules were intensely reactive for acid phosphatase activity, while the inner Golgi saccules were reactive for thiamine pyrophosphatase and nucleoside diphosphatase activities. In the rat exorbital lacrimal and parotid glands, reaction product for endogenous peroxidase, a secretory enzyme, was present in the endoplasmic reticulum, Golgi saccules, immature and mature secretory granules. GERL was usually free of reaction product or contained only a small amount. The widespread occurrence of GERL in secretory cells, and its intimate involvement with the formation of granules, suggest that it is an integral component of the secretory process.
在几种不同的外分泌腺细胞中研究了GERL的结构和细胞化学，这些细胞包括大鼠的眶外泪腺、腮腺、舌浆液腺（冯·埃布纳腺）、颌下腺和舌下腺以及外分泌胰腺；豚鼠的泪腺、腮腺和胰腺；以及猴的泪腺。在所研究的所有细胞类型中，GERL在形态学和细胞化学上都相似。它位于高尔基体内区，由扁平囊和管状部分组成。未成熟的分泌颗粒通过多个管状连接与GERL相连。仅在一个表面有核糖体的内质网变形扁平囊与GERL的部分紧密平行。GERL和未成熟颗粒对酸性磷酸酶活性呈强烈反应，而高尔基体内侧扁平囊对硫胺素焦磷酸酶和核苷二磷酸酶活性呈反应。在大鼠眶外泪腺和腮腺中，作为一种分泌酶的内源性过氧化物酶的反应产物存在于内质网、高尔基扁平囊、未成熟和成熟的分泌颗粒中。GERL通常没有反应产物或仅含有少量反应产物。GERL在分泌细胞中的广泛存在及其与颗粒形成的密切关系表明它是分泌过程的一个组成部分。
