Hase K, Kadota S, Basnet P, Takahashi T, Namba T
Research Institute for Wakan-Yaku, Toyama Medical and Pharmaceutical University, Japan.
Biol Pharm Bull. 1996 Apr;19(4):567-72. doi: 10.1248/bpb.19.567.
Hepatoprotective effect of celosian, an acidic polysaccharide isolated from the water extract of the seed of Celosia argentea, was investigated using chemical and immunological liver injury models. Celosian inhibited the elevation of serum enzyme (GPT, GOT, LDH) and bilirubin levels on carbon tetrachloride (CC1(4))-induced liver injuries in rat. In addition, the hepatoprotective effect of celosian was also observed in this model of liver injury by histopathological findings. Moreover, celosian suppressed rises in GPT or mortality on fulminant hepatitis induced by D-galactosamine/lipopolysaccharide (D-Ga1N/LPS) or Propionibacterium acnes/LPS in mice. These findings suggested that celosian is an active component in protection against chemical and immunological hepatitis and the activity was found to be a dose dependent. Celosian showed a concentration dependent inhibitory effect on lipid peroxide (LPO) generation in vitro. Though celosian did not reduce the release of tumor necrosis factor-alpha (TNF-alpha), it protected against recombinant human TNF-alpha (rhTNF-alpha)-induced liver injury in D-galactosamine sensitized mice.
使用化学和免疫性肝损伤模型，对从青葙种子水提取物中分离出的一种酸性多糖——青葙多糖的肝脏保护作用进行了研究。青葙多糖可抑制四氯化碳（CC1₄）诱导的大鼠肝损伤中血清酶（谷丙转氨酶、谷草转氨酶、乳酸脱氢酶）和胆红素水平的升高。此外，通过组织病理学检查结果，在该肝损伤模型中也观察到了青葙多糖的肝脏保护作用。而且，青葙多糖可抑制小鼠由D-半乳糖胺/脂多糖（D-Ga1N/LPS）或痤疮丙酸杆菌/LPS诱导的暴发性肝炎中谷丙转氨酶的升高或死亡率。这些发现表明，青葙多糖是预防化学性和免疫性肝炎的活性成分，且该活性呈剂量依赖性。青葙多糖在体外对脂质过氧化物（LPO）的生成显示出浓度依赖性抑制作用。虽然青葙多糖并未降低肿瘤坏死因子-α（TNF-α）的释放，但它可保护D-半乳糖胺致敏小鼠免受重组人TNF-α（rhTNF-α）诱导的肝损伤。
