Williams P, Keshavarz-Moore E, Dunnill P
Department of Chemical and Biochemical Engineering, University College, London, UK.
J Biotechnol. 1996 Jul 31;48(3):259-67. doi: 10.1016/0168-1656(96)01520-9.
Cadmium sulphate was added to separate batch cultures of Schizosaccharomyces pombe during different growth phases to determine the effect on cadmium sulphide microcrystallite production. Exit gas analysis was used to determine the impact on metabolism. Addition during the early-exponential growth phase resulted in an immediate intracellular uptake of cadmium, followed by rapid efflux from the cells, permanent reduction in cell metabolism and a lower intracellular inorganic sulphide content. This response was not suitable for cadmium sulphide microcrystallite production. Stationary phase cultures did not induce cadmium sulphide microcrystallite production. However, the addition of cadmium sulphate to a culture during the mid-exponential growth phase increased the intracellular cadmium and inorganic sulphide concentrations for approximately 8 h before reaching a saturation level for the cell. This resulted in a significant level of cadmium sulphide microcrystallite production.
在不同生长阶段，将硫酸镉添加到粟酒裂殖酵母的单独分批培养物中，以确定其对硫化镉微晶产生的影响。通过尾气分析来确定其对代谢的影响。在指数生长早期添加硫酸镉，会导致镉立即被细胞内摄取，随后迅速从细胞中流出，细胞代谢永久性降低，细胞内无机硫化物含量降低。这种反应不适合用于硫化镉微晶的产生。稳定期培养物不会诱导硫化镉微晶的产生。然而，在指数生长中期向培养物中添加硫酸镉，会使细胞内镉和无机硫化物浓度在约8小时内增加，直至达到细胞饱和水平。这导致了显著水平的硫化镉微晶产生。
