幽门螺杆菌抗原刺激下人类T淋巴细胞的增殖。

The proliferation of human T lymphocytes stimulated by Helicobacter pylori antigens.

作者信息

Chmiela M, Paziak-Domanska B, Ljungh A, Wadström T, Rudnicka W

机构信息

Department of Medical Microbiology, Lund University, Sweden.

出版信息

Immunobiology. 1996 Jul;195(2):199-208. doi: 10.1016/s0171-2985(96)80039-8.

DOI:10.1016/s0171-2985(96)80039-8

PMID:8877396

Abstract

Fractionated mononuclear cells (MNCs) were obtained from peripheral blood of healthy human volunteers, seronegative for H. pylori antibodies. The MNCs were stimulated in culture with whole live or heat-killed H. pylori cells or with bacterial cell surface (SA) or cytoplasmic (CA) antigens. There was a marked proliferative response of T cells in cultures stimulated with 10(5) cells/well of live H. pylori, 5 micrograms/well of CA or 5-20 micrograms/well of SA. However, no proliferation was observed in MNC cultures containing higher "doses" of live H. pylori organisms (10(7)/well) or CA (20 micrograms/well). Moreover, higher "doses" of the bacteria or CA entirely inhibited the response of T cells to PHA.

摘要

从幽门螺杆菌抗体血清阴性的健康人类志愿者外周血中获取分离的单核细胞（MNC）。将MNC在培养物中用全活或热灭活的幽门螺杆菌细胞或细菌细胞表面（SA）或细胞质（CA）抗原进行刺激。在用每孔10(5)个活幽门螺杆菌细胞、每孔5微克CA或每孔5 - 20微克SA刺激的培养物中，T细胞有明显的增殖反应。然而，在含有更高“剂量”活幽门螺杆菌生物体（每孔10(7)个）或CA（每孔20微克）的MNC培养物中未观察到增殖。此外，更高“剂量”的细菌或CA完全抑制了T细胞对PHA的反应。