Wegener H C, Baggesen D L
Danish Zoonosis Centre, Danish Veterinary Laboratory, Copenhagen, Denmark.
Int J Food Microbiol. 1996 Sep;32(1-2):125-31. doi: 10.1016/0168-1605(96)01114-2.
Analysis of chromosomal DNA restriction patterns produced by pulsed field gel electrophoresis (PFGE) was used to investigate an outbreak of human salmonellosis caused by Salmonella enterica ssp. enterica serovar Infantis (S. Infantis) involving more than 500 registered human cases. The outbreak had been tentatively traced back to a single pig slaughterhouse. A total of 135 isolates from various sources produced 21 different PFGE patterns with the restriction endonuclease XbaI. All human isolates from the outbreak belonged to a single type, the 'EPI-type', whereas human isolates recovered before and after the outbreak belonged to several different types. All isolates investigated from the suspect pig slaughterhouse and its supplier pig herds belonged to the EPI-type. Isolates from pork from the central meat market in Copenhagen, which received most of the carcasses from the suspect slaughterhouse, also belonged to the EPI-type. This was furthermore, the case for isolates from beef from the same market, indicating that cross-contamination had taken place. All isolates from pork and some, but not all, isolates from beef, collected in butchers' shops during the outbreak belonged to the EPI-type. The typing results supported that the outbreak was a common source outbreak, probably originating from a limited number of supplier pig herds supplying animals to a single slaughterhouse.
采用脉冲场凝胶电泳（PFGE）分析染色体DNA限制性酶切图谱，以调查由肠炎沙门氏菌亚种肠炎血清型婴儿沙门氏菌（S. Infantis）引起的一起人类沙门氏菌病暴发，该疫情涉及500多例登记在案的人类病例。此次暴发初步追溯至一家单一的生猪屠宰场。从不同来源共分离出135株菌株，用限制性内切酶XbaI产生了21种不同的PFGE图谱。疫情期间所有人类分离株均属于单一类型，即“EPI型”，而疫情前后分离出的人类菌株则属于几种不同类型。从可疑生猪屠宰场及其供应猪群中调查的所有分离株均属于EPI型。哥本哈根中央肉类市场的猪肉分离株也属于EPI型，该市场接收了大部分来自可疑屠宰场的胴体。同样，来自同一市场牛肉的分离株也是如此，表明发生了交叉污染。疫情期间在肉店收集的所有猪肉分离株以及部分（但并非全部）牛肉分离株均属于EPI型。分型结果支持此次暴发是一次共同来源暴发，可能源自向单一屠宰场供应动物的有限数量的供应猪群。
