Carceles C M, Espuny A, Vicente M S, Diaz M S, Escudero E
Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, University of Murcia, Spain.
Res Vet Sci. 1996 Sep;61(2):143-6. doi: 10.1016/s0034-5288(96)90089-2.
The pharmacokinetic behaviour of a combination of ampicillin and sulbactam (2:1) in six sheep and six goats after single intravenous doses of 20 mg kg body weight-1 (13.33 mg kg-1 of ampicillin and 6.67 mg kg-1 of sulbactam) was investigated by using a high-performance liquid chromatographic method for determining plasma concentrations. The objective was to determine whether there are differences between sheep and goats in the disposition kinetics of ampicillin and sulbactam. The plasma concentration-time curves were analysed by compartmental pharmacokinetic and non-compartmental methods. The disposition curves for both drugs were best described by a biexponential equation (two-compartment open model) in both sheep and goats. The mean (SD) elimination half-lives of ampicillin were 0.32 (0.05) h in sheep and 0.32 (0.04) h in goats, and the half-lives of sulbactam were 0.74 (0.10) h and 0.79 (0.18) h in sheep and goats, respectively. The apparent volumes of distribution of ampicillin and sulbactam were similar in the two species. Mean (SD) body clearances of ampicillin were 0.69 (0.07) litre h-1 kg-1 in sheep and 0.72 (0.11) litre h-1 kg-1 in goats, and the body clearances of sulbactam were 0.38 (0.03) and 0.38 (0.07) litre h-1 kg-1 in sheep and goats, respectively. There were no significant differences between any of the pharmacokinetic parameters of ampicillin and sulbactam in the sheep and goats.
采用高效液相色谱法测定血浆浓度，研究了6只绵羊和6只山羊单次静脉注射20mg/kg体重（其中氨苄西林13.33mg/kg，舒巴坦6.67mg/kg）后氨苄西林与舒巴坦（2:1）组合的药代动力学行为。目的是确定绵羊和山羊在氨苄西林和舒巴坦处置动力学方面是否存在差异。采用房室药代动力学和非房室方法分析血浆浓度-时间曲线。绵羊和山羊中两种药物的处置曲线均可用双指数方程（二室开放模型）进行最佳描述。氨苄西林在绵羊和山羊中的平均（标准差）消除半衰期分别为0.32（0.05）小时和0.32（0.04）小时，舒巴坦在绵羊和山羊中的半衰期分别为0.74（0.10）小时和0.79（0.18）小时。两种动物中氨苄西林和舒巴坦的表观分布容积相似。氨苄西林在绵羊和山羊中的平均（标准差）机体清除率分别为0.69（0.07）升/小时·千克和0.72（0.11）升/小时·千克，舒巴坦在绵羊和山羊中的机体清除率分别为0.38（0.03）和0.38（0.07）升/小时·千克。绵羊和山羊中氨苄西林和舒巴坦的任何药代动力学参数之间均无显著差异。
