Yildiz F H, Davies J P, Grossman A
Carnegie Institution of Washington, Department of Plant Biology, Stanford, California 94305, USA.
Plant Physiol. 1996 Oct;112(2):669-75. doi: 10.1104/pp.112.2.669.
A Chlamydomonas reinhardtii adenosine triphosphate (ATP) sulfurylase cDNA clone (pATS1) was selected by complementing a mutation in the ATP sulfurylase gene (cysD) of Escherichia coli. E. coli cysD strains harboring pATS1 grow on medium containing sulfate as the sole sulfur source and exhibit ATP sulfurylase activity. The amino acid sequence of the C. reinhardtii ATP sulfurylase, derived from the nucleotide sequence of the complementing gene (ATS1), is 25 to 40% identical to that of ATP sulfurylases in other eukaryotic organisms and has a putative transit peptide at its amino terminus. ATP sulfurylase mRNA was present when cells were grown in sulfur-replete medium, but accumulated to higher levels when the cells were exposed to sulfur-limiting conditions. Furthermore, sulfur-stress-induced accumulation of the ATS1 transcript was reduced in a strain defective in SAC1, a gene that is critical for acclimation to sulfur-limited growth.
通过互补大肠杆菌ATP硫酸化酶基因（cysD）中的一个突变，筛选出了莱茵衣藻腺苷三磷酸（ATP）硫酸化酶cDNA克隆（pATS1）。携带pATS1的大肠杆菌cysD菌株在以硫酸盐作为唯一硫源的培养基上生长，并表现出ATP硫酸化酶活性。从互补基因（ATS1）的核苷酸序列推导得到的莱茵衣藻ATP硫酸化酶的氨基酸序列，与其他真核生物中的ATP硫酸化酶的氨基酸序列有25%至40%的同一性，并且在其氨基末端有一个推定的转运肽。当细胞在硫充足的培养基中生长时，存在ATP硫酸化酶mRNA，但当细胞暴露于硫限制条件时，其积累水平更高。此外，在SAC1基因缺陷的菌株中，硫胁迫诱导的ATS1转录本积累减少，SAC1基因对于适应硫限制生长至关重要。
