Lin C W, Moorefield B, Payne J, Aprikian P, Mitomo K, Reeder R H
Basic Sciences Division, Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
Mol Cell Biol. 1996 Nov;16(11):6436-43. doi: 10.1128/MCB.16.11.6436.
We report the cloning of RRN11, a gene coding for a 66-kDa protein essential for transcription initiation by RNA polymerase I (Pol I) in the yeast Saccharomyces cerevisiae. Rrn11 specifically complexes with two previously identified transcription factors, Rrn6 and Rrn7 (D. A. Keys, J. S. Steffan, J. A. Dodd, R. T. Yamamoto, Y. Nogi, and M. Nomura, Genes Dev. 8:2349-2362, 1994). The Rrn11-Rrn6-Rrn7 complex also binds the TATA-binding protein and is required for transcription by the core domain of the Pol I promoter. Therefore, we have designated the Rrn11-Rrn6-Rrn7-TATA-binding protein complex the yeast Pol I core factor. A two-hybrid assay was used to demonstrate involvement of short leucine heptad repeats on both Rrn11 and Rrn6 in the in vivo association of these two proteins. This assay also verified the previously described strong association between Rrn6 and Rrn7, independent of the Rrn6 leucine repeat.
我们报道了RRN11基因的克隆，该基因编码一种66 kDa的蛋白质，对酿酒酵母中RNA聚合酶I（Pol I）转录起始至关重要。Rrn11特异性地与两个先前鉴定的转录因子Rrn6和Rrn7结合（D. A. Keys、J. S. Steffan、J. A. Dodd、R. T. Yamamoto、Y. Nogi和M. Nomura，《基因与发育》8:2349 - 2362，1994）。Rrn11 - Rrn6 - Rrn7复合物也结合TATA结合蛋白，并且是Pol I启动子核心结构域转录所必需的。因此，我们将Rrn11 - Rrn6 - Rrn7 - TATA结合蛋白复合物命名为酵母Pol I核心因子。利用双杂交试验证明了Rrn11和Rrn6上的短亮氨酸七肽重复序列参与了这两种蛋白质在体内的结合。该试验还证实了先前描述的Rrn6和Rrn7之间的强结合，不依赖于Rrn6亮氨酸重复序列。
