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RNA聚合酶I介导的不依赖ATP的转录起始的结构机制

Structural mechanism of ATP-independent transcription initiation by RNA polymerase I.

作者信息

Han Yan, Yan Chunli, Nguyen Thi Hoang Duong, Jackobel Ashleigh J, Ivanov Ivaylo, Knutson Bruce A, He Yuan

机构信息

Department of Molecular Biosciences, Northwestern University, Evanston, United States.

Department of Chemistry, Georgia State University, Atlanta, United States.

出版信息

Elife. 2017 Jun 17;6:e27414. doi: 10.7554/eLife.27414.

DOI:10.7554/eLife.27414
PMID:28623663
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5489313/
Abstract

Transcription initiation by RNA Polymerase I (Pol I) depends on the Core Factor (CF) complex to recognize the upstream promoter and assemble into a Pre-Initiation Complex (PIC). Here, we solve a structure of Pol I-CF-DNA to 3.8 Å resolution using single-particle cryo-electron microscopy. The structure reveals a bipartite architecture of Core Factor and its recognition of the promoter from -27 to -16. Core Factor's intrinsic mobility correlates well with different conformational states of the Pol I cleft, in addition to the stabilization of either Rrn7 N-terminal domain near Pol I wall or the tandem winged helix domain of A49 at a partially overlapping location. Comparison of the three states in this study with the Pol II system suggests that a ratchet motion of the Core Factor-DNA sub-complex at upstream facilitates promoter melting in an ATP-independent manner, distinct from a DNA translocase actively threading the downstream DNA in the Pol II PIC.

摘要

RNA聚合酶I（Pol I）的转录起始依赖于核心因子（CF）复合物来识别上游启动子并组装成预起始复合物（PIC）。在此，我们使用单颗粒冷冻电子显微镜将Pol I-CF-DNA的结构解析到3.8 Å的分辨率。该结构揭示了核心因子的二分结构及其对从-27到-16的启动子的识别。核心因子的固有流动性与Pol I裂隙的不同构象状态密切相关，此外，Rrn7 N端结构域在Pol I壁附近或A49的串联翼状螺旋结构域在部分重叠位置的稳定也与之相关。本研究中这三种状态与Pol II系统的比较表明，上游核心因子-DNA亚复合物的棘轮运动以不依赖ATP的方式促进启动子解链，这与在Pol II PIC中主动穿入下游DNA的DNA转位酶不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/18ae167e7c7a/elife-27414-resp-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/adf9a1d1a850/elife-27414-fig1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/18ae167e7c7a/elife-27414-resp-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/adf9a1d1a850/elife-27414-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/6cc9c093f116/elife-27414-fig1-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/b8478289949e/elife-27414-fig1-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/428e4ee36904/elife-27414-fig1-figsupp3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/6045c6ed5803/elife-27414-fig1-figsupp4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/650eb3dd8d31/elife-27414-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/ee5ad02d87ba/elife-27414-fig2-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/1862e9e7f915/elife-27414-fig2-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/056f4556855e/elife-27414-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/cbcc19626e6a/elife-27414-fig3-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/2d0b0cbfc5fa/elife-27414-fig3-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/bc327042bcd5/elife-27414-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/aba00df98aec/elife-27414-fig4-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/dceae788a1cb/elife-27414-fig4-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/5e0f11a1699b/elife-27414-fig4-figsupp3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/5491180ea048/elife-27414-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/24502610faec/elife-27414-fig5-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/75c2c8e9457c/elife-27414-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/4dbe1f267c9e/elife-27414-fig6-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37fc/5489313/18ae167e7c7a/elife-27414-resp-fig1.jpg

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