厌氧培养的大肠杆菌细胞中NADH脱氢酶I和二甲基亚砜还原酶的H⁺/e⁻化学计量学
H+/e- stoichiometry for NADH dehydrogenase I and dimethyl sulfoxide reductase in anaerobically grown Escherichia coli cells.
作者信息
Bogachev A V, Murtazina R A, Skulachev V P
机构信息
Department of Bioenergetics, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.
出版信息
J Bacteriol. 1996 Nov;178(21):6233-7. doi: 10.1128/jb.178.21.6233-6237.1996.
Abstract
Anaerobically grown Escherichia coli cells were shown to acidify the reaction medium in response to oxygen or dimethyl sulfoxide (DMSO) pulses, with the H+/e- stoichiometry being close to 2.5 and 1.5, respectively. In the presence of the NADH dehydrogenase I (NDH-I) inhibitor 8-methyl-N-vanillyl-6-nonenamide (capsaicin) or in mutants lacking NDH-I, this ratio decreased to 1 for O2 and to 0 for DMSO. These data suggest that (i) the H+/e- stoichiometry for E. coli NDH-I is at least 1.5 and (ii) the DMSO reductase does not generate a proton motive force.
摘要
厌氧培养的大肠杆菌细胞在氧气或二甲基亚砜（DMSO）脉冲刺激下会使反应培养基酸化，H⁺/e⁻化学计量比分别接近2.5和1.5。在存在NADH脱氢酶I（NDH-I）抑制剂8-甲基-N-香草基-6-壬酰胺（辣椒素）时，或在缺乏NDH-I的突变体中，该比例对于氧气降至1，对于DMSO降至0。这些数据表明：（i）大肠杆菌NDH-I的H⁺/e⁻化学计量比至少为1.5；（ii）DMSO还原酶不会产生质子动力。
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