Kornelisse R F, Sluijter M, Spanjaard L, Hermans P W, de Groot R
Department of Pediatrics, Sophia Children's Hospital/University Hospital Rotterdam, The Netherlands.
Eur J Clin Microbiol Infect Dis. 1996 Aug;15(8):635-8. doi: 10.1007/BF01691148.
The molecular epidemiological characteristics of pneumococcal meningitis in children were studied. Pneumococcal isolates were characterized by serotyping and two genotyping methods, BOX fingerprinting and restriction fragment end labeling, to evaluate whether clonal strains were present within the serotypes or serogroups. During a 17-year period, 68 children admitted to the Sophia Children's Hospital were diagnosed with meningitis due to Streptococcus pneumoniae. Pneumococcal isolates from 44 patients were still available for analysis. All strains were susceptible to penicillin. Serotypes/ serogroups 14, 19, 6, and 18 represented 56% of all isolates. The results of the molecular typing methods demonstrate the absence of clonal relatedness between isolates from patients with pneumococcal meningitis.
对儿童肺炎球菌性脑膜炎的分子流行病学特征进行了研究。通过血清分型和两种基因分型方法（BOX指纹图谱法和限制性片段末端标记法）对肺炎球菌分离株进行特征分析，以评估血清型或血清群中是否存在克隆菌株。在17年期间，索菲亚儿童医院收治的68例儿童被诊断为肺炎链球菌性脑膜炎。来自44例患者的肺炎球菌分离株仍可用于分析。所有菌株对青霉素均敏感。血清型/血清群14、19、6和18占所有分离株的56%。分子分型方法的结果表明，肺炎球菌性脑膜炎患者的分离株之间不存在克隆相关性。
