Gérard C, Bruyns C, Marchant A, Abramowicz D, Vandenabeele P, Delvaux A, Fiers W, Goldman M, Velu T
Institut de Recherche Interdisciplinaire, Université Libre de Bruxelles, Belgium.
J Exp Med. 1993 Feb 1;177(2):547-50. doi: 10.1084/jem.177.2.547.
Because of its ability to efficiently inhibit in vitro cytokine production by activated macrophages, we hypothesized that interleukin (IL) 10 might be of particular interest in preventing endotoxin-induced toxicity. We therefore examined the effects of IL-10 administration before lipopolysaccharide (LPS) challenge in mice. A marked reduction in the amounts of LPS-induced tumor necrosis factor (TNF) release in the circulation was observed after IL-10 pretreatment at doses at low as 10 U. IL-10 also efficiently prevented the hypothermia generated by the injection of 100 micrograms LPS. Finally, pretreatment with a single injection of 1,000 U IL-10 completely prevented the mortality consecutive to the challenge with 500 micrograms LPS, a dose that was lethal in 50% of the control mice. We conclude that IL-10 inhibits in vivo TNF secretion and protects against the lethality of endotoxin in a murine model of septic shock.
由于白细胞介素（IL）-10能够有效抑制体外活化巨噬细胞产生细胞因子，我们推测IL-10在预防内毒素诱导的毒性方面可能具有特殊意义。因此，我们研究了在小鼠脂多糖（LPS）攻击前给予IL-10的效果。在低至10 U剂量的IL-10预处理后，观察到循环中LPS诱导的肿瘤坏死因子（TNF）释放量显著减少。IL-10还能有效预防注射100微克LPS所产生的体温过低。最后，单次注射1000 U IL-10预处理完全预防了500微克LPS攻击后的死亡，该剂量在50%的对照小鼠中是致命的。我们得出结论，在脓毒症休克小鼠模型中，IL-10可抑制体内TNF分泌并保护机体免受内毒素致死作用。
