Vullo T, Deo-Narine V, Stallmeyer M J, Gomez D G, Cahill P T
Department of Radiology, New York Hospital--Cornell University Medical College, NY 10021, USA.
Magn Reson Imaging. 1996;14(6):657-62. doi: 10.1016/0730-725x(95)02083-6.
The hippocampal formation possesses an important role in the development and maintenance of short-term memory. In this study, magnetic resonance imaging (MRI) and gross histology were used to quantify the volume of the hippocampal formation in canines. High resolution MRI, using 1 mm thick slices and an intraplanar resolution of 0.35 mm was performed at 2.0 T both in vivo and in vitro following in situ fixation. The volumes of the hippocampal formations were determined from MR images and compared to those obtained from one mm thick gross histologic sections. The average volume of the canine hippocampal formation, measured from in vivo and in vitro MR images was 476.0 +/- 79.5 and 467.3 +/- 53.7 mm3, respectively. Determined from gross histology, the volume of the hippocampal formation was 463.6 +/- 24.1 mm3. Quantitation of the canine hippocampal formation using in vivo MRI showed good correlation with in vitro MRI and histology, verifying the reliability and reproducibility of in vivo MRI measurements. High resolution MRI using 1 mm thick slices through the whole canine hippocampal formation is necessary for accurate volume determination of a structure of this size.
海马结构在短期记忆的形成和维持中起着重要作用。在本研究中，使用磁共振成像（MRI）和大体组织学方法对犬类海马结构的体积进行量化。在2.0 T条件下，对原位固定后的犬类进行了体内和体外高分辨率MRI检查，切片厚度为1 mm，平面分辨率为0.35 mm。从MR图像中确定海马结构的体积，并与从1 mm厚的大体组织切片中获得的体积进行比较。从体内和体外MR图像测量得到的犬类海马结构的平均体积分别为476.0 +/- 79.5和467.3 +/- 53.7 mm3。通过大体组织学确定，海马结构的体积为463.6 +/- 24.1 mm3。使用体内MRI对犬类海马结构进行定量分析，结果显示与体外MRI和组织学具有良好的相关性，证实了体内MRI测量的可靠性和可重复性。使用1 mm厚的切片对整个犬类海马结构进行高分辨率MRI检查，对于准确确定这种大小结构的体积是必要的。
