Florian C L, Williams S R, Bhakoo K K, Noble M D
The Royal College of Surgeons Unit of Biophysics, Institute of Child Health, London, United Kingdom.
Neurochem Res. 1996 Sep;21(9):1065-74. doi: 10.1007/BF02532417.
Regional and developmental changes in metabolite concentrations were measured by 1H NMR spectroscopy and HPLC of perchloric acid extracts from rat brain and eye. The highest concentrations of N-acetylaspartate were found in grey matter as opposed to white matter with concentration increasing with age from neonate to adult, while the related compound N-acetylaspartylglutamate was highest in adult optic nerve. Creatine and choline-containing compounds were present in all regions throughout development, with higher levels of creatine found in grey matter compared to other regions. Choline-containing compounds were present at the highest concentrations in the eye at all ages examined, and tended to decrease in concentration to minimum values in adulthood in all regions. The presence of hypotaurine in corpus callosum and optic nerve was consistent with the metabolic profiles of O-2A progenitor cells and oligodendrocytes, which are cells composing these tissues. The neurotransmitters glutamate and GABA reached their highest concentrations in the olfactory bulb (higher than in adult cortex).
通过对大鼠脑和眼的高氯酸提取物进行1H核磁共振波谱分析和高效液相色谱分析，测量了代谢物浓度的区域和发育变化。与白质相比，灰质中N-乙酰天门冬氨酸的浓度最高，且从新生儿到成年，其浓度随年龄增长而增加，而相关化合物N-乙酰天门冬氨酰谷氨酸在成年视神经中含量最高。在整个发育过程中，所有区域都存在肌酸和含胆碱化合物，与其他区域相比，灰质中的肌酸水平更高。在所检查的所有年龄段中，含胆碱化合物在眼中的浓度最高，并且在所有区域中，其浓度在成年期往往会降至最低值。胼胝体和视神经中牛磺酸的存在与O-2A祖细胞和少突胶质细胞的代谢谱一致，这些细胞构成了这些组织。神经递质谷氨酸和γ-氨基丁酸在嗅球中的浓度达到最高（高于成年皮质）。
