Langfort J, Czarnowski D, Pilis W, Wójcik B, Górski J
Department of Applied Physiology, Medical Research Centre, Polish Academy of Sciences, Warszawa, Poland.
Biochem Mol Med. 1996 Oct;59(1):28-32. doi: 10.1006/bmme.1996.0060.
The aim of the present study was to find out whether activities of the enzymes controlling adenosine metabolism, 5'-nucleotidase (5NT) and adenosine deaminase (ADA), in the left ventricle of the rat's heart change after 6 weeks of endurance or sprint training. Additionally, an influence of a single bout of endurance exercise till exhaustion on activities of these enzymes was investigated in sedentary and trained rats. The rats were divided into three groups: (1) sedentary controls (C), (2) endurance-trained (ET), and (3) sprint-trained (ST). It was shown that both types of training increased 5NT, but did not change ADA activity in the rat heart. Acute exercise till exhaustion did not affect 5NT activity in the heart taken from C and ST rats, but decreased its activity in the ET group. The heart ADA activity after exhaustive exercise increased in C and in ET group, but decreased in ST animals. It is concluded that physical training affects cardiac adenosine metabolism and the type of training may exert an influence on purine nucleotides metabolism in the heart during exhaustive exercise.
本研究的目的是探究大鼠心脏左心室中控制腺苷代谢的酶，即5'-核苷酸酶（5NT）和腺苷脱氨酶（ADA）的活性在进行6周耐力训练或短跑训练后是否会发生变化。此外，还研究了在久坐不动和经过训练的大鼠中，单次耐力运动直至力竭对这些酶活性的影响。大鼠被分为三组：（1）久坐对照组（C），（2）耐力训练组（ET），以及（3）短跑训练组（ST）。结果表明，两种类型的训练均使大鼠心脏中的5NT增加，但并未改变ADA的活性。急性运动直至力竭对C组和ST组大鼠心脏中的5NT活性没有影响，但使ET组大鼠心脏中的5NT活性降低。力竭运动后，C组和ET组大鼠心脏中的ADA活性增加，但ST组动物心脏中的ADA活性降低。结论是，体育锻炼会影响心脏的腺苷代谢，并且训练类型可能在力竭运动期间对心脏中的嘌呤核苷酸代谢产生影响。
