暴露于交通尾气中的路边蚯蚓体内污染金属的亚细胞定位
Subcellular localization of polluting metals in roadside earthworms exposed to traffic exhaust gases.
作者信息
Gullvåg B M
出版信息
Cytobios. 1978;22(87-88):141-53.
The aim was to make a subcellular localization of metals in tissue from lumbricid earthworms exposed to environmental pollution. Scanning transmission electron microscopy in combination with energy dispersive X-ray microanalysis of tissue fixed in glutaraldehyde only, and with no electron staining, was used. Zinc was registered in the metachromatic mucous granules of the epidermal cells, and zinc, iron and lead in the chloragosomes of the chloragocytes suggesting that metal may be excreted together with the slime or stored in chloragosomes. Relatively few metal nuclear inclusions were encountered probably due to the fact that some metal leaks out during the preparation process. A comparison is made with a chemical analysis of cellular fractions (Talberg, 1977).
目的是对暴露于环境污染中的蚯蚓组织中的金属进行亚细胞定位。采用仅用戊二醛固定且不进行电子染色的组织进行扫描透射电子显微镜结合能量色散X射线微分析。锌存在于表皮细胞的异染性粘液颗粒中，锌、铁和铅存在于氯细胞的黄色细胞中，这表明金属可能与粘液一起排出或储存在黄色细胞中。由于在制备过程中一些金属会泄漏，因此遇到的金属核内包涵体相对较少。与细胞组分的化学分析进行了比较（塔尔贝格，1977年）。
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