分子技术在微孢子虫感染诊断中的应用。
Application of molecular techniques to the diagnosis of microsporidial infection.
作者信息
Fedorko D P, Hijazi Y M
机构信息
National Institutes of Health, Bethesda, Maryland 20892-1508, USA.
出版信息
Emerg Infect Dis. 1996 Jul-Sep;2(3):183-91. doi: 10.3201/eid0203.960304.
Abstract
Microsporidia are now recognized as important pathogens of AIDS patients; the ability of these parasites to cause disease in immunocompetent persons is still being elucidated. Improved diagnostic tests for microsporidial infection are continually being sought for establishing diagnosis in order to avoid laborious electron microscopy studies that require invasively acquired biopsy specimens. Modified trichrome or chemofluorescent stains are useful for detecting microsporidia in bodily fluids and stool specimens, but they do not allow for speciation of microsporidia. Polymerase chain reaction with specific primers will allow the detection and speciation of microsporidia in biopsy tissue, bodily fluids, and stool specimens.
摘要
微孢子虫现在被认为是艾滋病患者的重要病原体；这些寄生虫在免疫功能正常者中引发疾病的能力仍在研究之中。为了避免需要通过侵入性获取活检标本的繁琐电子显微镜研究，人们一直在寻求改进的微孢子虫感染诊断测试以进行确诊。改良的三色染色法或化学荧光染色法对于检测体液和粪便标本中的微孢子虫很有用，但它们无法对微孢子虫进行分类。使用特异性引物的聚合酶链反应能够检测活检组织、体液和粪便标本中的微孢子虫并对其进行分类。
相似文献
1
Application of molecular techniques to the diagnosis of microsporidial infection.分子技术在微孢子虫感染诊断中的应用。
Emerg Infect Dis. 1996 Jul-Sep;2(3):183-91. doi: 10.3201/eid0203.960304.
2
Microsporidiosis: molecular and diagnostic aspects.微孢子虫病：分子与诊断方面
Adv Parasitol. 1998;40:351-95. doi: 10.1016/s0065-308x(08)60127-x.
3
Improved light-microscopical detection of microsporidia spores in stool and duodenal aspirates. The Enteric Opportunistic Infections Working Group.粪便和十二指肠抽吸物中微孢子虫孢子的光镜检测方法改进。肠道机会性感染工作组。
N Engl J Med. 1992 Jan 16;326(3):161-6. doi: 10.1056/NEJM199201163260304.
4
Detection of Microsporidia by different staining techniques.采用不同染色技术检测微孢子虫。
J Egypt Soc Parasitol. 1998 Dec;28(3):729-38.
5
[New parasitic diseases in man: infections caused by Microsporida and Cyclospora species].[人类中的新型寄生虫病：由微孢子虫属和环孢子虫属物种引起的感染]
Schweiz Med Wochenschr. 1995 May 6;125(18):909-23.
6
7
[Contribution of Trichrome Blue in the diagnosis of microsporidiosis].[三色蓝在微孢子虫病诊断中的作用]
Bull Soc Pathol Exot. 1996;89(3):179-80.
8
Intestinal microsporidiosis.肠道微孢子虫病
Semin Gastrointest Dis. 1997 Jan;8(1):45-55.
9
Microsporidial infections in humans: current practice and developments in laboratory diagnosis.人类微孢子虫感染：实验室诊断的当前实践与进展
Br J Biomed Sci. 1997 Sep;54(3):216-21.
10
A powerful DNA extraction method and PCR for detection of microsporidia in clinical stool specimens.一种用于检测临床粪便标本中微孢子虫的高效DNA提取方法及聚合酶链反应
Clin Diagn Lab Immunol. 1999 Mar;6(2):243-6. doi: 10.1128/CDLI.6.2.243-246.1999.
引用本文的文献
1
Nosema Disease of European Honey Bees.欧洲蜜蜂微孢子虫病
J Fungi (Basel). 2021 Aug 30;7(9):714. doi: 10.3390/jof7090714.
2
Alternatives in Molecular Diagnostics of and Infections.分子诊断中针对[具体感染名称缺失]感染的替代方法。
J Fungi (Basel). 2020 Jul 22;6(3):114. doi: 10.3390/jof6030114.
3
A novel application of small-angle scattering techniques: Quality assurance testing of virus quantification technology.小角散射技术的一种新应用：病毒定量技术的质量保证测试。
Radiat Phys Chem Oxf Engl 1993. 2008 Mar;77(3):215-224. doi: 10.1016/j.radphyschem.2007.10.004. Epub 2007 Oct 23.
4
Therapeutic targets for the treatment of microsporidiosis in humans.人类微孢子虫病治疗的靶点。
Expert Opin Ther Targets. 2018 Nov;22(11):903-915. doi: 10.1080/14728222.2018.1538360. Epub 2018 Nov 1.
5
Comparative evaluation of staining techniques and polymerase chain reaction for diagnosis of intestinal microsporidiosis in immunocompromised patients.免疫功能低下患者肠道微孢子虫病诊断中染色技术与聚合酶链反应的比较评估
Trop Parasitol. 2015 Jul-Dec;5(2):101-5. doi: 10.4103/2229-5070.162491.
6
Identification of Encephalitozoon and Enterocytozoon (microsporidia) spores in stool and urine samples obtained from free-living South American Coatis (Nasua nasua).从自由生活的南美的浣熊（Nasua nasua）的粪便和尿液样本中鉴定出脑炎微孢子虫和肠微孢子虫（微孢子虫）孢子。
Appl Environ Microbiol. 2012 Jun;78(12):4490-2. doi: 10.1128/AEM.07708-11. Epub 2012 Apr 20.
7
DNA detection and genotypic identification of potentially human-pathogenic microsporidia from asymptomatic pet parrots in South Korea as a risk factor for zoonotic emergence.从韩国无症状宠物鹦鹉中检测和鉴定潜在的人致病性微孢子虫的 DNA 及其基因型，作为人畜共患病出现的危险因素。
Appl Environ Microbiol. 2011 Dec;77(23):8442-4. doi: 10.1128/AEM.05343-11. Epub 2011 Sep 30.
8
Molecular identification and genotyping of Microsporidia in selected hosts.对选定宿主中的微孢子虫进行分子鉴定和基因分型。
Parasitol Res. 2012 Feb;110(2):689-93. doi: 10.1007/s00436-011-2543-9. Epub 2011 Jul 20.
9
Molecular diagnostic tests for microsporidia.微孢子虫的分子诊断测试
Interdiscip Perspect Infect Dis. 2009;2009:926521. doi: 10.1155/2009/926521. Epub 2009 Aug 2.
10
Application of transmission electron microscopy to the clinical study of viral and bacterial infections: present and future.透射电子显微镜在病毒和细菌感染临床研究中的应用：现状与未来
Micron. 2006;37(2):91-106. doi: 10.1016/j.micron.2005.10.001. Epub 2005 Nov 2.
本文引用的文献
1
A case of Encephalitozoon-like body infection in man.一例人类似脑炎微孢子虫体感染病例。
AMA Arch Pathol. 1959 Feb;67(2):181-7.
2
Identification and characterization of three Encephalitozoon cuniculi strains.三种兔脑炎微孢子虫菌株的鉴定与特性分析
Parasitology. 1995 Nov;111 ( Pt 4):411-21. doi: 10.1017/s0031182000065914.
3
Quantitative light microscopic detection of Enterocytozoon bieneusi in stool specimens: a longitudinal study of human immunodeficiency virus-infected microsporidiosis patients.粪便标本中微小隐孢子虫的定量光学显微镜检测：对人类免疫缺陷病毒感染的微孢子虫病患者的纵向研究
J Clin Microbiol. 1996 Mar;34(3):520-3. doi: 10.1128/jcm.34.3.520-523.1996.
4
Immunologic and molecular characteristics of Encephalitozoon-like microsporidia isolated from humans and rabbits indicate that Encephalitozoon cuniculi is a zoonotic parasite.从人类和兔子体内分离出的类脑炎微孢子虫的免疫学和分子特征表明，兔脑炎微孢子虫是一种人畜共患寄生虫。
Clin Infect Dis. 1996 Mar;22(3):557-9. doi: 10.1093/clinids/22.3.557.
5
Sensitive PCR diagnosis of Infections by Enterocytozoon bieneusi (microsporidia) using primers based on the region coding for small-subunit rRNA.基于编码小亚基核糖体RNA区域的引物对微小隐孢子虫（微孢子虫）感染进行灵敏的PCR诊断
J Clin Microbiol. 1996 Apr;34(4):986-7. doi: 10.1128/jcm.34.4.986-987.1996.
6
Diagnosis of disseminated microsporidian Encephalitozoon hellem infection by PCR-Southern analysis and successful treatment with albendazole and fumagillin.通过聚合酶链反应- Southern分析诊断播散性微小孢子虫海伦脑炎微孢子虫感染，并使用阿苯达唑和烟曲霉素成功治疗。
J Clin Microbiol. 1996 Apr;34(4):947-52. doi: 10.1128/jcm.34.4.947-952.1996.
7
Encephalocytozoon intestinalis-specific monoclonal antibodies for laboratory diagnosis of microsporidiosis.用于微孢子虫病实验室诊断的肠脑炎微孢子虫特异性单克隆抗体。
J Clin Microbiol. 1996 Feb;34(2):282-5. doi: 10.1128/jcm.34.2.282-285.1996.
8
Effects of nifedipine, metronidazole, and nitric oxide donors on spore germination and cell culture infection of the microsporidia Encephalitozoon hellem and Encephalitozoon intestinalis.硝苯地平、甲硝唑和一氧化氮供体对微小孢子虫脑孢子虫和肠孢子虫孢子萌发及细胞培养感染的影响。
Antimicrob Agents Chemother. 1996 Jan;40(1):179-85. doi: 10.1128/AAC.40.1.179.
9
Comparison of three staining methods for detecting microsporidia in fluids.三种检测体液中微孢子虫染色方法的比较
J Clin Microbiol. 1995 Dec;33(12):3138-45. doi: 10.1128/jcm.33.12.3138-3145.1995.
10
Secondary structure of the small subunit ribosomal RNA sequence of the microsporidium Encephalitozoon cuniculi.兔脑炎微孢子虫小亚基核糖体RNA序列的二级结构
Nucleic Acids Res. 1993 Mar 25;21(6):1489. doi: 10.1093/nar/21.6.1489.
Zotero 插件