Guerreiro P, Barreiros T, Soares H, Cyrne L, Maia e Silva A, Rodrigues-Pousada C
Laboratorio de Genetica Molecular, Instituto Gulbenkian de Ciencia, Oeiras Portugal.
Yeast. 1996 Mar 15;12(3):273-80. doi: 10.1002/(SICI)1097-0061(19960315)12:3%3C273::AID-YEA898%3E3.0.CO;2-1.
A 17.6 kb DNA fragment from the right arm of chromosome VII of Saccharomyces cerevisiae has been sequenced and analysed. The sequence contains twelve open reading frames (ORFs) longer than 100 amino acids. Three genes had already been cloned and sequenced: CCT, ADE3 and TR-I. Two ORFs are similar to other yeast genes: G7722 with the YAL023 (PMT2) and PMT1 genes, encoding two integral membrane proteins, and G7727 with the first half of the genes encoding elongation factors 1gamma, TEF3 and TEF4. Two other ORFs, G7742 and G7744, are most probably yeast orthologues of the human and Paracoccus denitrificans electron-transferring flavoproteins (beta chain) and of the Escherichia coli phosphoserine phosphohydrolase. The five remaining identified ORFs do not show detectable homology with other protein sequences deposited in data banks. The sequence has been deposited in the EMBL data library under Accession Number Z49133.
已对酿酒酵母七号染色体右臂上一段17.6 kb的DNA片段进行了测序和分析。该序列包含十二个开放阅读框(ORF),其长度超过100个氨基酸。已有三个基因被克隆和测序:CCT、ADE3和TR-I。两个开放阅读框与其他酵母基因相似:G7722与YAL023(PMT2)和PMT1基因相似,这两个基因编码两种整合膜蛋白;G7727与编码延伸因子1γ、TEF3和TEF4的基因的前半部分相似。另外两个开放阅读框G7742和G7744很可能是人类和反硝化副球菌电子传递黄素蛋白(β链)以及大肠杆菌磷酸丝氨酸磷酸水解酶的酵母直系同源物。其余五个已鉴定的开放阅读框与数据库中存储的其他蛋白质序列未显示出可检测到的同源性。该序列已存入EMBL数据库,登录号为Z49133。