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对酿酒酵母十五号染色体右臂上51千碱基对进行测序和分析,发现了30个开放阅读框。

Sequencing and analysis of 51 kb on the right arm of chromosome XV from Saccharomyces cerevisiae reveals 30 open reading frames.

作者信息

Wiemann S, Rechmann S, Benes V, Voss H, Schwager C, Vlcek C, Stegemann J, Zimmermann J, Erfle H, Paces V, Ansorge W

机构信息

Biochemical Instrumentation, EMBL, Heidelberg, Germany.

出版信息

Yeast. 1996 Mar 15;12(3):281-8. doi: 10.1002/(SICI)1097-0061(19960315)12:3%3C281::AID-YEA904%3E3.0.CO;2-O.

Abstract

We have sequenced a region of 51 kb of the right arm from chromosome XV of Saccharomyces cerevisiae. The sequence contains 30 open reading frames (ORFs) of more than 100 amino acid residues. Thirteen new genes have been identified. Thirteen ORFs correspond to known yeast genes. One delta element and one tRNA gene were identified. Upstream of the RPO31 gene, encoding the largest subunit of RNA polymerase III, lies a Abf1p binding site. The nucleotide sequence data reported in this paper are available in the EMBL, GenBank and DDBJ nucleotide sequence databases under the Accession Number X90518.

摘要

我们对酿酒酵母第十五号染色体右臂上51 kb的区域进行了测序。该序列包含30个开放阅读框(ORF),其氨基酸残基超过100个。已鉴定出13个新基因。13个ORF与已知的酵母基因相对应。鉴定出一个δ元件和一个tRNA基因。在编码RNA聚合酶III最大亚基的RPO31基因上游,存在一个Abf1p结合位点。本文报道的核苷酸序列数据可在EMBL、GenBank和DDBJ核苷酸序列数据库中获取,登录号为X90518。

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