Happonen I, Saari S, Castren L, Tyni O, Hänninen M L, Westermarck E
Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, Helsinki University, Finland.
J Comp Pathol. 1996 Aug;115(2):117-27. doi: 10.1016/s0021-9975(96)80034-x.
Diagnostic methods for detecting gastric Helicobacter-like organisms (GHLOs) in dogs and cats were compared. Samples for brush cytology, the urease test and histological examination were collected post mortem from the fundus, corpus and antrum of 10 dogs (17 sample sites from each animal) and 10 cats (14 sample sites each). Samples of tissue from the fundus or corpus were taken for transmission electron microscopy and culture from three and eight dogs, respectively, and from six cats that gave a positive urease test with samples from these regions. In all dogs and in six of the 10 cats, GHLOs were detected by at least one of three methods (brush cytology, urease test or histological examination) in all regions. By brush cytology, GHLOs were demonstrated in all samples from the dogs and the positive cats. In cats, the urease test (60 min) gave a positive result in every sample site; in dogs it gave a positive result in 100% of the corpus samples, in 95% of the fundus samples and in 62% of the antral samples. Histological examination revealed GHLOs in all samples from the fundus and corpus of the dogs and of the positive cats; and in 74% and 91.7% of the antral samples of the dogs and cats, respectively. GHLOs were seen in all dogs and cats studied by transmission electron microscopy, and culture of gastric tissue was successful in 3/8 dogs and 1/6 cats. In this study, brush cytology was thus the most sensitive method for demonstrating GHLOs.
对检测犬猫胃幽门螺杆菌样微生物（GHLOs）的诊断方法进行了比较。通过刷片细胞学、尿素酶试验和组织学检查的样本在死后从10只犬（每只动物17个采样部位）和10只猫（每只14个采样部位）的胃底、胃体和胃窦采集。分别从3只犬和8只犬以及6只尿素酶试验呈阳性的猫的胃底或胃体采集组织样本用于透射电子显微镜检查和培养，这些样本来自这些区域。在所有犬以及10只猫中的6只中，通过三种方法（刷片细胞学、尿素酶试验或组织学检查）中的至少一种在所有区域检测到了GHLOs。通过刷片细胞学，在犬和阳性猫的所有样本中均证实存在GHLOs。在猫中，尿素酶试验（60分钟）在每个采样部位均呈阳性结果；在犬中，胃体样本100%呈阳性结果，胃底样本95%呈阳性结果，胃窦样本62%呈阳性结果。组织学检查显示，犬和阳性猫的胃底和胃体的所有样本中均存在GHLOs；犬和猫的胃窦样本中分别有74%和91.7%存在GHLOs。通过透射电子显微镜检查在所有研究的犬和猫中均观察到了GHLOs，胃组织培养在8只犬中的3只和6只猫中的1只成功。因此，在本研究中，刷片细胞学是证明GHLOs最敏感的方法。
