Mathur A, Michalowicz B, Castillo M, Aeppli D
Department of Oral Sciences, University of Minnesota, Minneapolis 55455, USA.
J Periodontal Res. 1996 Oct;31(7):489-95. doi: 10.1111/j.1600-0765.1996.tb01414.x.
Cytokines play an important role in the pathology associated with chronic inflammatory diseases. We measured the total amounts [picograms (pg)] and concentrations.(pg/microliter) of interleukin-1 alpha (IL-1 alpha), interleukin-8 (IL-8) and interferon-alpha (IFN-alpha) in 20 s gingival crevicular fluid (GCF) samples obtained from 2 diseased and 2 healthy sites in 20 subjects with periodontitis, and from 2 healthy sites in 20 subjects without disease. Both the mean amount and concentration of IL-1 alpha were significantly higher (p < 0.001) in diseased sites compared to healthy sites in subjects with disease. The results for IL-8 and IFN-alpha differed depending on the method of reporting. Whereas the amount of IL-8 was significantly higher (p < 0.01) in diseased sites, the mean concentration of IL-8 was lower compared to healthy sites. The mean amount of IFN-alpha was similar in health and disease; however, the concentration of IFN-alpha was significantly lower in diseased sites (p < 0.001) corresponding to the significant increase in crevicular fluid volume (p < 0.001). There were no significant differences in the amount or concentrations of the 3 cytokines between healthy sites from subjects with disease and healthy sites from healthy controls. The total amounts of both IFN-alpha and IL-8 were correlated between healthy and diseased sites in subjects. These data suggest that, while the disease status of a site is the major determinant of the levels of these cytokines locally, subjects with high levels of IL-8 and IFN-alpha in healthy sites also tend to have high levels of these cytokines in diseased sites. Finally, both the concentrations and total amounts of IL-8 and IFN-alpha were significantly correlated in diseased sites, suggesting that levels of these two cytokines rise or fall in tandem. The combination of decreased IL-8 and decreased IFN-alpha concentrations at diseased sites may reflect the reduced anti-bacterial host defense activity at that site.
细胞因子在与慢性炎症性疾病相关的病理学中发挥着重要作用。我们测量了从20名患有牙周炎的受试者的2个患病部位和2个健康部位以及20名无疾病受试者的2个健康部位采集的20份龈沟液（GCF）样本中白细胞介素-1α（IL-1α）、白细胞介素-8（IL-8）和干扰素-α（IFN-α）的总量[皮克（pg）]和浓度（pg/微升）。与患病受试者的健康部位相比，患病部位IL-1α的平均量和浓度均显著更高（p < 0.001）。IL-8和IFN-α的结果因报告方法而异。虽然患病部位IL-8的量显著更高（p < 0.01），但其平均浓度低于健康部位。IFN-α的平均量在健康和患病状态下相似；然而，患病部位IFN-α的浓度显著更低（p < 0.001），这与龈沟液体积的显著增加相对应（p < 0.001）。患病受试者的健康部位与健康对照的健康部位之间这3种细胞因子的量或浓度没有显著差异。受试者健康部位和患病部位的IFN-α和IL-8总量均具有相关性。这些数据表明，虽然局部这些细胞因子水平的主要决定因素是部位的疾病状态，但健康部位IL-8和IFN-α水平高的受试者在患病部位这些细胞因子的水平往往也较高。最后，患病部位IL-8和IFN-α的浓度和总量均显著相关，表明这两种细胞因子的水平同步上升或下降。患病部位IL-8和IFN-α浓度降低的组合可能反映了该部位抗菌宿主防御活性的降低。
