Riggio M P, Macfarlane T W, Mackenzie D, Lennon A, Smith A J, Kinane D
Infection and Immunity Research-Group, Glasgow Dental Hospital and School, Scotland, UK.
J Periodontal Res. 1996 Oct;31(7):496-501. doi: 10.1111/j.1600-0765.1996.tb01415.x.
In this study, the major periodontal pathogens Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis were detected in subgingival plaque samples from patients with periodontal disease by polymerase chain reaction (PCR) and conventional culture methods. 170 plaque samples from 43 patients were analysed; A. actinomycetemcomitans and P. gingivalis were each detected in 40 (24%) of samples by PCR, whereas conventional culture methods detected A. actinomycetemcomitans and P. gingivalis in 25 (15%) and 18 (11%) of samples, respectively. The proportion of patients carrying A. actinomycetemcomitans in at least 1 sampled periodontal site was 17/43 (40%) by PCR and 13/43 (30%) by culture; for P. gingivalis this was 12/43 (28%) by PCR and 9/43 (21%) by culture. Only 5 samples, from 3 patients, harboured both A. actinomycetemcomitans and P. gingivalis. It is concluded that PCR is more accurate than conventional culture methods for identification of these periodontal pathogens in subgingival plaque samples and has a higher frequency of detection.
在本研究中，通过聚合酶链反应（PCR）和传统培养方法，在牙周病患者的龈下菌斑样本中检测到主要的牙周病原体伴放线放线杆菌和牙龈卟啉单胞菌。对43例患者的170份菌斑样本进行了分析；通过PCR在40份（24%）样本中分别检测到伴放线放线杆菌和牙龈卟啉单胞菌，而传统培养方法分别在25份（15%）和18份（11%）样本中检测到伴放线放线杆菌和牙龈卟啉单胞菌。通过PCR检测，在至少1个采样牙周部位携带伴放线放线杆菌的患者比例为17/43（40%），通过培养为13/43（30%）；对于牙龈卟啉单胞菌，通过PCR为12/43（28%），通过培养为9/43（21%）。只有来自3例患者的5份样本同时含有伴放线放线杆菌和牙龈卟啉单胞菌。得出的结论是，在龈下菌斑样本中，PCR在鉴定这些牙周病原体方面比传统培养方法更准确，且检测频率更高。
