在绵羊肝片吸虫病实验性感染和自然感染中循环巨片形吸虫抗原的检测

Detection of circulating Fasciola gigantica antigen in experimental and natural infections of sheep with fasciolosis.

作者信息

Guobadia E E, Fagbemi B O

机构信息

Department of Veterinary Microbiology and Parasitology, University of Ibadan, Nigeria.

出版信息

Vet Parasitol. 1996 Oct 15;65(1-2):29-39. doi: 10.1016/0304-4017(96)00937-5.

DOI:10.1016/0304-4017(96)00937-5

PMID:8916398

Abstract

Detection of circulating Fasciola gigantica antigen was performed in sera of sheep with experimental and natural F. gigantica infections using the direct enzyme-linked immunosorbent assay method. Sera from sheep with monoinfections of Schistosoma bovis, Dicrocoelium hospes and Paramphistomum microbothrium were included in the assay to ascertain specificity. Circulating F. gigantica antigen (CFA) was detected as early as 1 week after infection in the experimentally infected sheep. No detectable CFA was observed 2 weeks after chemotherapy. Positivity rates of 82.5%, 12.5%, 10% and 10% were found in sera with monospecific infections of F. gigantica, P. microbrothrium, D. hospes and S. bovis, respectively. Acid treatment of the sera did not enhance the sensitivity of the assay.

摘要

采用直接酶联免疫吸附测定法，对感染了实验性和自然感染的巨片形吸虫的绵羊血清进行循环巨片形吸虫抗原检测。试验中纳入了感染牛血吸虫、霍氏双腔吸虫和微小片形吸虫单感染的绵羊血清，以确定特异性。在实验感染的绵羊中，感染后1周即可检测到循环巨片形吸虫抗原（CFA）。化疗后2周未观察到可检测到的CFA。在分别感染巨片形吸虫、微小片形吸虫、霍氏双腔吸虫和牛血吸虫单特异性感染的血清中，阳性率分别为82.5%、12.5%、10%和10%。血清的酸处理并未提高检测的灵敏度。