Liu Y, Davin-Regli A, Bosi C, Charrel R N, Bollet C
Laboratoire d'Hygiène et de Microbiologie et Comité de Lutte contre l'Infection Nosocomiale, Hôpital Salvator, Marseille, France.
J Med Microbiol. 1996 Nov;45(5):359-65. doi: 10.1099/00222615-45-5-359.
Between July 1994 and March 1995, 64 isolates of Pseudomonas aeruginosa were implicated in bacteraemia in 25 cancer patients in five wards of two hospitals. These, together with 24 environmental isolates and one isolate from a bacteraemia in a non-cancer patient were examined by three PCR-based DNA fingerprinting methods: random amplified polymorphic DNA (RAPD), enterobacterial-repetitive intergenic consensus (ERIC)-PCR, and 16S-23S spacer region-based RAPD. These methods were reproducible, discriminatory and showed close agreement; all indicated that 47 isolates that had caused bacteraemia in 19 cancer patients were indistinguishable. Seventeen other isolates that had caused bacteraemia in 10 cancer patients were discriminated into eight further groups, and the 24 environmental and non-cancer patient isolates into further distinct groups. No environmental source of the epidemic strain was found, but it was suspected that the outbreak was related to infusion implants.
1994年7月至1995年3月期间，两家医院五个病房的25名癌症患者发生了64株铜绿假单胞菌菌血症。将这些菌株，连同24株环境分离株和1株非癌症患者菌血症的分离株，采用三种基于聚合酶链反应（PCR）的DNA指纹图谱方法进行检测：随机扩增多态性DNA（RAPD）、肠杆菌重复基因间共有序列（ERIC）-PCR和基于16S-23S间隔区的RAPD。这些方法具有可重复性、鉴别力，且结果高度一致；所有结果均表明，导致19名癌症患者发生菌血症的47株分离株无法区分。导致10名癌症患者发生菌血症的其他17株分离株被进一步分为8个组，24株环境和非癌症患者分离株则被分为更不同的组。未发现流行菌株的环境来源，但怀疑此次暴发与输液植入物有关。
