Ilouno L E, Shu E N, Igbokwe G E
Department of Applied Biochemistry, Nnamdi Azikwe University, Awka, Nigeria.
Clin Chim Acta. 1996 Mar 29;247(1-2):1-6. doi: 10.1016/0009-8981(95)06193-2.
Superoxide dismutase (SOD) activity of erythrocytes from normal pregnant women (controls) and from pre-eclamptic toxaemia (PET) cases was determined by the method of Winterbourn et al. (J Lab Clin Med 1975;85:337-341) with slight modifications. Instead of the Michaelis-Menten type of plot that is usually obtained and which these authors have found to be imprecise, a linear plot was obtained when the reciprocal values for the percentage inhibition of nitroblue tetrazolium (NBT) reduction were plotted against the corresponding volumes of red blood cell (RBC) extract. This plot was found to give precise values for the volumes of RBC extracts required for 50% inhibition of NBT reduction. Consequently, a clearer understanding is beginning to emerge regarding the SOD activity of PET cases compared with that of normal pregnant women.
采用Winterbourn等人（《实验室与临床医学杂志》1975年；85卷：337 - 341页）的方法并略作修改，测定了正常孕妇（对照组）和先兆子痫病例红细胞中的超氧化物歧化酶（SOD）活性。当将抑制氮蓝四唑（NBT）还原的百分比的倒数与相应体积的红细胞（RBC）提取物绘制在一起时，得到的是线性图，而不是通常得到的米氏类型的图，这些作者发现米氏类型的图不精确。发现该图能给出50%抑制NBT还原所需的RBC提取物体积的精确值。因此，与正常孕妇相比，先兆子痫病例的SOD活性开始有了更清晰的认识。
