Butterton J R, Ryan E T, Shahin R A, Calderwood S B
Infectious Disease Unit, Massachusetts General Hospital, Boston 02114, USA.
Infect Immun. 1996 Oct;64(10):4373-7. doi: 10.1128/iai.64.10.4373-4377.1996.
A mouse model of Vibrio cholerae infection was successfully developed with germfree mice. Three- to four-week-old germfree mice were orally inoculated with strains of V. cholerae to be tested and then moved to normal housing after inoculation. Stool culture, measurement of serum vibriocidal antibody titers, and determination of immune responses to the cholera toxin B subunit demonstrated that germfree mice are readily colonized by V cholerae and develop systemic and mucosal immune responses to antigens expressed by these organisms. Immune responses to the B subunit of Shiga toxin 1, which was expressed from a V. cholerae vaccine vector, were less pronounced. This model should be valuable for studying immune responses to V. cholerae infection and immunization, including responses to heterologous antigens expressed by cholera vector strains.
利用无菌小鼠成功建立了霍乱弧菌感染的小鼠模型。将3至4周龄的无菌小鼠经口接种待测试的霍乱弧菌菌株，接种后转移至正常饲养环境。粪便培养、血清杀弧菌抗体滴度测定以及对霍乱毒素B亚单位的免疫反应测定表明，无菌小鼠很容易被霍乱弧菌定殖，并对这些细菌表达的抗原产生全身和黏膜免疫反应。对由霍乱弧菌疫苗载体表达的志贺毒素1 B亚单位的免疫反应则不太明显。该模型对于研究霍乱弧菌感染和免疫的免疫反应，包括对霍乱载体菌株表达的异源抗原的反应应该是有价值的。
