VanEtten C H, Daxenbichler M E
J Assoc Off Anal Chem. 1977 Jul;60(4):946-9.
Details are given for determining total glucosinolates in Cruciferae plants by a procedure measuring released glucose. The glucosinolates are separated from about 90% of other material in the plant extract by adsorption on an anion exchange resin. Then, by a selective thioglucosidase hydrolysis of the glucosinolates retained on the exchange resin, the glucose and aglucons are separated from other substances retained by the resin. Glucose is released into an aqueous medium and is equivalent to the total glucosinolates. The aglucons formed by the hydrolysis are extracted into methylene chloride and determined by gas-liquid chromatography. Based on 29 determinations of the glucose from sinigrin, analyzed under different conditions, accuracy of the total glucosinolate determination was 94.8 +/- 7.3%. The coefficient of variation, determined by duplicate analyses on extracts from 58 cabbage samples, was 4.6%.
给出了通过测量释放的葡萄糖的方法来测定十字花科植物中总硫代葡萄糖苷的详细信息。硫代葡萄糖苷通过吸附在阴离子交换树脂上，从植物提取物中约90%的其他物质中分离出来。然后，通过对保留在交换树脂上的硫代葡萄糖苷进行选择性硫代葡萄糖苷酶水解，将葡萄糖和糖苷配基与树脂保留的其他物质分离。葡萄糖释放到水介质中，其含量相当于总硫代葡萄糖苷的含量。水解形成的糖苷配基被萃取到二氯甲烷中，并通过气液色谱法进行测定。基于在不同条件下对黑芥子硫苷葡萄糖进行的29次测定，总硫代葡萄糖苷测定的准确度为94.8±7.3%。通过对58个卷心菜样品提取物进行重复分析确定的变异系数为4.6%。
