Daxenbichler M E, VanEtten C H
J Assoc Off Anal Chem. 1977 Jul;60(4):950-3.
A procedure is described for identifying and quantitatively determining the glucosinolates found in edible cabbage. The intact glucosinolates are extracted and their aglucons are released into solvent after a special enzymatic hydrolysis step. The resulting isothiocyanates and oxazolidinethiones are separated by a gas-liquid chromatographic procedure that allows the individual aglucons to be identified and their amounts to be estimated by comparison with an internal reference.
本文描述了一种用于鉴定和定量测定食用卷心菜中硫代葡萄糖苷的方法。完整的硫代葡萄糖苷经提取后，经过一个特殊的酶水解步骤，其糖苷配基释放到溶剂中。生成的异硫氰酸酯和恶唑烷硫酮通过气液色谱法进行分离，该方法能够鉴定各个糖苷配基，并通过与内标物比较来估算其含量。
