Xu Y, Zhaori G, Vene S, Shen K, Zhou Y, Magnius L O, Wahren B, Linde A
Department of Virology, Swedish Institute for Infectious Disease Control, Stockholm, Sweden.
Pediatr Infect Dis J. 1996 Nov;15(11):1018-24. doi: 10.1097/00006454-199611000-00017.
To understand the viral etiology of acute childhood encephalitis in Beijing.
Ninety-seven Chinese children (between 7 months and 13 years of age) with acute encephalitis were retrospectively investigated. They were treated in Beijing Children's Hospital between June, 1991, and October, 1994. Different serologic methods (immunofluorescence assay, enzyme-linked immunosorbent assay, solid phase reverse immunosorbent test) were used for detection of IgM antibody to enteroviruses, herpesviruses, mumps, measles, rubella and Japanese encephalitis virus. The viral DNA of six herpesviruses was detected by polymerase chain reaction.
Viral etiology was identified in 35 of 97 (36.0%) cases. The most frequently identified pathogens were enteroviruses (15; 15.4%), followed by mumps (7; 7.2%), rubella (6; 6.1%), Japanese encephalitis virus (5; 5.1%), human herpesvirus 6 (2; 2.0%), herpes simplex virus (2; 2.0%) and Epstein-Barr virus (1; 1.0%). IgM antibody in cerebrospinal fluid was detected for enterovirus, mumps and rubella viruses.
Enteroviruses were the most frequent viral pathogens of acute childhood encephalitis in Beijing. Detection of IgM in cerebrospinal fluid may be useful for diagnosis in certain cases of viral encephalitis.
了解北京儿童急性脑炎的病毒病因。
回顾性调查了97名中国儿童（年龄在7个月至13岁之间），他们于1991年6月至1994年10月在北京儿童医院接受治疗。采用不同的血清学方法（免疫荧光测定、酶联免疫吸附测定、固相反向免疫吸附试验）检测肠道病毒、疱疹病毒、腮腺炎病毒、麻疹病毒、风疹病毒和日本脑炎病毒的IgM抗体。通过聚合酶链反应检测六种疱疹病毒的病毒DNA。
97例病例中有35例（36.0%）确定了病毒病因。最常鉴定出的病原体是肠道病毒（15例；15.4%），其次是腮腺炎病毒（7例；7.2%）、风疹病毒（6例；6.1%）、日本脑炎病毒（5例；5.1%）、人疱疹病毒6型（2例；2.0%）、单纯疱疹病毒（2例；2.0%）和EB病毒（1例；1.0%）。在脑脊液中检测到肠道病毒、腮腺炎病毒和风疹病毒的IgM抗体。
肠道病毒是北京儿童急性脑炎最常见的病毒病原体。脑脊液中IgM的检测在某些病毒性脑炎病例的诊断中可能有用。
