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针对大鼠肉瘤的单克隆抗体。I. 杂交瘤生产的免疫程序及淋巴细胞来源

Monoclonal antibodies to rat sarcomata. I. Immunization procedures and source of lymphoid cells for hybridoma production.

作者信息

North S M, Styles J M, Hobbs S M, Dean C J

出版信息

Immunology. 1982 Nov;47(3):397-405.

PMID:7129525
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1555540/
Abstract

Hybridomas secreting monoclonal antibodies with specificity for Hooded rat fibrosarcomata have been obtained by fusion of the rat myeloma Y3 Ag 1.2.3. (Galfre, Milstein & Wright, 1979) with cells taken from spleens or lymph nodes of immunized syngeneic and allogeneic donors. Two of the monoclonal antibodies, both derived from he spleens of tumour bearers, showed specificity for individual tumours one for MC24 (M10/76) and the other for HSNTC (11/160). These two antibodies had a long half-life in the blood when injected intravenously showing that they had a low affinity for normal tissue antigens. Monoclonal antibodies exhibiting broad tumour specificity or extensive cross-reactivity with normal cells were secreted by many of the hybridomas derived from both syngeneic and allogeneic rats that had been hyperimmunized with tumour cells. These results are discussed in relation to the production of monoclonal anti-tumour antibodies for use in experimental therapy.

摘要

通过将大鼠骨髓瘤Y3 Ag 1.2.3.（加尔弗雷、米尔斯坦和赖特，1979年）与取自免疫的同基因和异基因供体的脾脏或淋巴结的细胞融合，获得了对带帽大鼠纤维肉瘤具有特异性的分泌单克隆抗体的杂交瘤。两种单克隆抗体均来源于肿瘤携带者的脾脏，对单个肿瘤具有特异性，一种针对MC24（M10/76），另一种针对HSNTC（11/160）。静脉注射时，这两种抗体在血液中的半衰期很长，表明它们对正常组织抗原的亲和力较低。许多用肿瘤细胞超免疫的同基因和异基因大鼠来源的杂交瘤分泌出对肿瘤具有广泛特异性或与正常细胞有广泛交叉反应的单克隆抗体。本文讨论了这些结果与用于实验治疗的单克隆抗肿瘤抗体生产的关系。

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本文引用的文献

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THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.高比放射性碘-131标记人生长激素的制备
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Immunotoxins: hybrid molecules of monoclonal antibodies and a toxin subunit specifically kill tumour cells.免疫毒素：单克隆抗体与毒素亚基的杂交分子可特异性杀死肿瘤细胞。
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