Majima K
Department of Ophthalmology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
Ophthalmic Surg Lasers. 1996 Nov;27(11):946-51.
The degree of cell adhesion to intraocular lenses (IOLs) was studied through the cell culture system and the morphology of adhering cells was studied using a light electron microscope and a scanning electron microscope.
Human lens epithelial cells were used as materials. The IOL materials were classified into four groups: polymethylmethacrylate (PMMA), heparin surface modified PMMA, surface passivated PMMA, and silicone.
PMMA showed greater adhesion than did the other materials. Cells hardly adhered to silicone. Use of a light electron microscope and a scanning electron microscope revealed that cells adhered uniformly to the surface of PMMA and were elongated, but did not adhere uniformly to IOLs of other materials. Only a few cells without the tendency of elongation were noted on the silicone the second day after culture. The 14th day after culture, the surfaces of IOLs, except those of silicone, were covered with cells.
Such differences in the surface adhesion and morphology of cells adhering to the IOL surface are important in evaluating IOL biocompatibility.
通过细胞培养系统研究细胞对人工晶状体（IOL）的黏附程度，并使用光学电子显微镜和扫描电子显微镜研究黏附细胞的形态。
以人晶状体上皮细胞为材料。将IOL材料分为四组：聚甲基丙烯酸甲酯（PMMA）、肝素表面改性的PMMA、表面钝化的PMMA和硅酮。
PMMA表现出比其他材料更强的黏附性。细胞几乎不黏附于硅酮。使用光学电子显微镜和扫描电子显微镜观察发现，细胞均匀地黏附于PMMA表面且呈伸长状，但未均匀地黏附于其他材料的IOL上。培养第二天，在硅酮上仅观察到少数无伸长趋势的细胞。培养第14天，除硅酮外的IOL表面均被细胞覆盖。
细胞在IOL表面的黏附及形态上的这些差异对于评估IOL生物相容性很重要。
