Kurland C, Gallant J
Department of Molecular Biology, Uppsala University, Box 590, Uppsala, S751 24, Sweden.
Curr Opin Biotechnol. 1996 Oct;7(5):489-93. doi: 10.1016/s0958-1669(96)80050-4.
Missense substitutions and processivity errors in the translation of heterologous proteins are expected to occur at higher frequencies than the corresponding errors of normal translation. The resulting error-containing products may overload chaperone systems. Likewise, there may be a risk of an immunogenic response to heterologous proteins introduced into vertebrates. Recent work has been carried out on the mechanisms by which such errors arise and on their occurrence in cloned, heterologous gene products.
与正常翻译的相应错误相比，预计在异源蛋白质翻译过程中错义替换和持续性错误出现的频率更高。由此产生的含错误产物可能会使伴侣系统不堪重负。同样，引入脊椎动物体内的异源蛋白质可能存在引发免疫反应的风险。最近针对此类错误产生的机制及其在克隆的异源基因产物中的发生情况开展了相关研究。
