Kuzuya M, Fujii R, Hamano M, Nakamura J, Yamada M, Nii S, Mori T
Department of Microbiology, Okayama Prefectural Institute for Environmental Science and Public Health, Japan.
J Clin Microbiol. 1996 Dec;34(12):3185-9. doi: 10.1128/jcm.34.12.3185-3189.1996.
Nucleotide sequences for the VP7 gene of human group C rotavirus were determined for two strains isolated in Okayama, Japan, during a 1988-1990 epidemic. These isolates, OK118 and OK450, were selected as prototypes of two different electropherotypes, patterns I and II, respectively. The genes were identical in size (1,063 bp), and both contained singled open reading frames encoding 332 amino acids. The alignment of two sequences revealed 46 nucleotide substitutions, 11 of which were predicted to give amino acid changes. The deduced amino acid sequence of VP7 from OK118 was similar to published sequences of a Japanese isolate and three foreign isolates (more than 98.4% identity), whereas the VP7 sequence of OK450 revealed around 96% identity with these isolates and had nine unique amino acid substitutions. The VP7 genes of nine Okayama isolated were than analyzed by dot blot hybridization with the VP7 probes of OK118 and OK450. Under highly stringent conditions, the OK118 probe produced strong hybridization signals with the genes of five pattern I strains and one pattern II strain, while the OK450 probe strongly reacted only with those of three pattern II strains. Our results concluded that relative sequence diversity in the VP7 gene was observed between two different electropherotypes prevalent in a limited area.
在1988 - 1990年日本冈山县的一次疫情期间，对两株人C组轮状病毒的VP7基因核苷酸序列进行了测定。这些分离株OK118和OK450分别被选为两种不同电泳型（模式I和模式II）的原型。这两个基因大小相同（1063 bp），均包含编码332个氨基酸的单一开放阅读框。两个序列的比对显示有46个核苷酸替换，其中11个预计会导致氨基酸变化。OK118的VP7推导氨基酸序列与一株日本分离株和三株国外分离株的已发表序列相似（同一性超过98.4%），而OK450的VP7序列与这些分离株的同一性约为96%，并有9个独特的氨基酸替换。然后用OK118和OK450的VP7探针通过斑点杂交分析了九株冈山县分离株的VP7基因。在高严格条件下，OK118探针与五株模式I菌株和一株模式II菌株的基因产生强杂交信号，而OK450探针仅与三株模式II菌株的基因强烈反应。我们的结果表明，在有限区域内流行的两种不同电泳型之间，VP7基因存在相对序列多样性。
