Korf J
Department of Biological Psychiatry, Groningen University, The Netherlands.
Dev Neurosci. 1996;18(5-6):405-14. doi: 10.1159/000111434.
We developed techniques to continuously monitor lactate in the living rat ('lactography') based on microdialysis and on-line enzymatic conversion of lactate in the dialysate using either continuous flow technologies or enzyme reactors. In vivo lactate was monitored during a single electroconvulsive shock, stress, swimming, repetitive hypoxia and after local infusion of probenecid (a blocker of carrier-mediated lactate transport) 1 or 2 days after implantation of the microdialysis probe. In a few experiments the rats were also perfused 1-2 weeks after surgery, so glial function predominates, because of gliosis around the probe. Our experiments indicate that extracellular lactate increases during stress, convulsion or exercise (particularly in the rat hippocampus), it is cleared from the extracellular space by a carrier-mediated mechanism and originates in glial cells at least in part. These in vivo studies support the concept of metabolic trafficking of lactate between glia cells and neurons.
我们基于微透析技术以及使用连续流动技术或酶反应器对透析液中的乳酸进行在线酶促转化，开发了在活体大鼠中连续监测乳酸的技术（“乳酸成像”）。在微透析探针植入后1或2天，于单次电惊厥休克、应激、游泳、重复性缺氧期间以及局部注入丙磺舒（一种载体介导的乳酸转运阻滞剂）后，对体内乳酸进行监测。在一些实验中，大鼠在手术后1 - 2周也进行了灌注，由于探针周围的胶质增生，此时神经胶质功能占主导。我们的实验表明，细胞外乳酸在应激、惊厥或运动期间增加（特别是在大鼠海马体中），它通过载体介导的机制从细胞外空间清除，并且至少部分起源于神经胶质细胞。这些体内研究支持了神经胶质细胞与神经元之间乳酸代谢转运的概念。
