子宫颈腺样囊性癌中整合型高危人乳头瘤病毒的检测
Detection of integrated high risk human papillomavirus in adenoid cystic carcinoma of the uterine cervix.
作者信息
Grayson W, Taylor L, Cooper K
机构信息
Department of Anatomical Pathology, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa.
出版信息
J Clin Pathol. 1996 Oct;49(10):805-9. doi: 10.1136/jcp.49.10.805.
AIM
To investigate the role of human papillomavirus (HPV) in adenoid cystic carcinoma of the uterine cervix.
METHODS
Eleven archival, paraffin wax embedded specimens were analysed by non-isotopic in situ hybridisation (NISH) for HPV types 6, 11, 16, 18, 31, and 33 using digoxigenin labelled probes. The polymerase chain reaction (PCR) was carried out on each of the cases using consensus primers to HPV.
RESULTS
A total of eight adenoid cystic carcinomas harboured the HPV genome by NISH, of which five were PCR positive. Integrated HPV 16 DNA was demonstrated in seven of the eight NISH positive cases. One adenoid cystic carcinoma showed integrated HPV 31. HPV DNA was not detected in the three remaining cases.
CONCLUSIONS
Integrated high risk HPV genome, in particular type 16, is associated with this uncommon type of primary cervical cancer.
目的
探讨人乳头瘤病毒（HPV）在子宫颈腺样囊性癌中的作用。
方法
采用地高辛标记探针，通过非同位素原位杂交（NISH）对11份存档石蜡包埋标本进行HPV 6、11、16、18、31和33型检测。对每个病例使用HPV通用引物进行聚合酶链反应（PCR）。
结果
通过NISH共检测出8例腺样囊性癌含有HPV基因组，其中5例PCR呈阳性。在8例NISH阳性病例中的7例检测到整合型HPV 16 DNA。1例腺样囊性癌显示整合型HPV 31。其余3例未检测到HPV DNA。
结论
整合型高危HPV基因组，尤其是16型，与这种罕见的原发性宫颈癌相关。
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